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Pass the loop through a flame 2 or 3 times for about 10 seconds at a time.

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Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?

A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.


Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?

Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.


Why is the loop heated before it is used to transfer bacteria?

The inoculating loop should be heated until it is hot enough to turn red, and then allowed to cool for a couple seconds. This ensures that the heat kills the majority of lingering bacteria before or after use.


How much do central sterile techs make?

Central sterile technicians make an average salary of around $35,000 to $50,000 per year, depending on location, experience, and employer.


Why shouldn't you dig into agar with the loop?

Streaking is to produce single colonies. If we are digging to the agar while streaking the microbes, instead of growing on the agar surface grows in the subsurface as well. These colonies may be difficult to isolate.

Related Questions

What are the uses of the Inoculating Loop?

Inoculating loops are used to transfer microorganisms to growth media or for staining slides. They are an important part of the sterile technique as their use permits transfer only of the material of interest.


Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?

A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.


Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?

Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.


Why is the loop heated before it is used to transfer bacteria?

The inoculating loop should be heated until it is hot enough to turn red, and then allowed to cool for a couple seconds. This ensures that the heat kills the majority of lingering bacteria before or after use.


Does depakote over time make you sterile?

I took depakote in the past and i would like to know if it will make you sterile. I now take tegretol and lamictal and i would like to know if tegretol or lamictal could make you sterile. If anyone has any answers on this i would like to know. My e-mail is hattmart@yahoo.com. Thank you


Can sleeping nude make a man sterile?

No this will not make him sterile.


Why do you grow bacteria on agar jelly?

Put agar jelly in a petri dish to sterilize the dish and then use something like a sterile inoculating loop to put the bacteria on the jelly. Then, seal the petri dish but make sure it is not airtight.


How will you be guaranteed not to get food poisoning?

The only way you could guarantee no food poisoning would be to sterilize all your food before eating, which would make most foods unpalatable. And to eat with sterile gloves and sterile utensils in a sterile environment.


Will male yeast infections make him sterile?

No but they will make his bread rise


Why should you let inoculating loop cool first before using?

an innoculated loop shouln't be hot. it should be cooled before contact with the organism by touching it off the edge of the agar or dipped into the top of the broth. if its hot it will kill the organism!


Is street light system is closed loop or open loop?

An open loop traffic signal just cycles on a schedule and has no "knowledge" of traffic patterns or current traffic. A closed loop system would have sensors letting it know when traffic was present and a controller that would make decisions based on the traffic conditions.


Are clones able to make offspring?

Unless they were cloned from sterile or hermaphroditic stock, I would say,. yes.