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The inoculating loop should be heated until it is hot enough to turn red, and then allowed to cool for a couple seconds. This ensures that the heat kills the majority of lingering bacteria before or after use.
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∙ 9y ago
Wiki User
∙ 13y agoUnless it's a nichrome loop or needle, it must be heated until it turns red, then wait for a few seconds to let it cool. If it's a disposable inoculating loop or needle, there is no need to heat because it is usually sterile already.
Wiki User
∙ 10y agoit must be heated to get rid of al the bacteria
Wiki User
∙ 8y agoBy heating the loop before using, all bacteria which may be on it will be destroyed. It is allowed to cool before using and it is sterilized again after use.
Wiki User
∙ 8y agoBecause too much heat can kill bacteria. Think of pasteurised milk - the pasteurising process involves rapid heating and cooling to kill off any bacteria.
Wiki User
∙ 10y agotoo make sure it is sterile
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What is the purpose of flaming the loop before and after use?
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
Why must you not flame your loop then quickly try to pick up bacteria with it?
The heat can kill the bacteria
Why are inoculating loop and needle flamed before and after use?
The heat from the flame destroys any bacteria on the wire loop. This prevents any transfer or cross contamination by unwanted or unintended bacteria. Put simply, this action thoroughly cleans the innoculating loop. To correctly perform this action, the loop is passed UP the column of flame from the bottom, where it is (relatively) cooler, to the TOP where the fully oxygenated gas is at its hottest temperature. Allow the loop to glow red hot for a few (2-3) seconds and then remove it from the flame. Allow a few seconds for the air to cool the loop, and it is again ready for its next use. Note: Small sparks may be seen to erupt as any bacterial cells burst into flame.
When is the loop preferable for transferring bacteria?
Subculturing refers to transferring microorganisms from one medium to another. For example, bacteria growing in broth may be transferred to an agar plate.Wire loops are used to transfer microorganisms from liquid media to liquid or solid media.Needles are used for transferring microorganisms to deep tubes.
Why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
Why is an wire loop heated in a Bunsen burner?
For sterilization purposes. The process of heating the loop to white hot kills all bacteria that remain between samples.
Why is loop flamed before it is placed in a culture tube?
To kill any bacteria on it.
Why that you should cool first the inculating loop or needle before using?
"because otherwise you will kill the bacteria from the heat, and you may also melt the agar jelly that you are innoculating the bacterium onto."
Why is the loop allowed to cool before putting it into the bacterial culture?
Because too much heat can kill bacteria. Think of pasteurised milk - the pasteurising process involves rapid heating and cooling to kill off any bacteria that might be present in the milk.
What is the purpose of flaming the loop before and after use?
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
Explain open-loop transfer function of a close system?
An open loop transfer function is the transfer function of a system without incorporating the feedback component. For example, where the closed loop transfer function with unity feedback H(s) = G(s)/(1+G(s)) it can be deduced that the open loop transfer function is Y(s)/U(s) = G(s).
What looping process checks the test condition at the end of the loop?
The do..while() loop tests the condition at the end of the loop. Therefore the loop body executes at least once. The while() loop (without do) tests the condition before entering the loop and before each iteration of the loop. The for() loop conditional expression is optional but, when specified, is tested before entering the loop and before each iteration of the loop.
Does a 'loop of water and air or loop of water transfer sound energy?
i don't know that is what i want to know
How do you prevent splattering when flamming a loop which has just been used to transfer a culture?
By sterilizing the loop by flaming it.
What is the meaning of loops?
of Loop, The running together of the matter of an ore into a mass, when the ore is only heated for calcination., of Loop.
What is the material that the loop used in bacterial smear make and why?
the loop is called innoculation loop, it is made up of nickel becase before looping a bacteria it needs to be sterilized in the flame, so nickel having high melting and bioling point and neutral in reactivity therefore it is used.
Why must you not flame your loop then quickly try to pick up bacteria with it?
The heat can kill the bacteria
