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What else can I help you with?
Why the loop is flamed after completing the inoculation?
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
Why the loop must be flamed before streaking a new group of lines?
the inoculation loop must be flamed before streaking a new group of line to avoid any type of contamination. This is said to be one type of sterilization(dry heat sterilization) process called incineration.
How would you make sure a inoculating loop was sterile?
To sterilize an inoculating loop, it can be flamed until it turns red hot. This process helps to kill any microorganisms that might be present. It is essential to allow the loop to cool before using it to avoid damaging the culture or injuring oneself.
When getting inoculums from a slant why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
Why are inoculating loop and needle flamed before and after use?
The heat from the flame destroys any bacteria on the wire loop. This prevents any transfer or cross contamination by unwanted or unintended bacteria. Put simply, this action thoroughly cleans the innoculating loop. To correctly perform this action, the loop is passed UP the column of flame from the bottom, where it is (relatively) cooler, to the TOP where the fully oxygenated gas is at its hottest temperature. Allow the loop to glow red hot for a few (2-3) seconds and then remove it from the flame. Allow a few seconds for the air to cool the loop, and it is again ready for its next use. Note: Small sparks may be seen to erupt as any bacterial cells burst into flame.
Why is it necessary to flame the loop between cultures?
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
How will you know when you have flamed the inoculating loop or needle long enough?
You will have flamed the inoculating loop or needle long enough when the entire wire or metal is glowing red-hot. This sterilizes the tool, ensuring that no contaminants are introduced into your sample.
Why does the quadrant steak technique able to give single colonies?
Isolation streaking yields isolated colonies by dilution. When the first zone is complete, the loop is flamed and cooled, and a small number of bacteria are dragged out of zone one to complete zone two. The loop is then flamed and cooled again, and a smaller number of bacteria are dragged out of zone two to complete zone three. The loop is flamed and cooled again, and a very small number of bacteria are pulled from zone three to complete zone four.
Why loop and cap should not be laid on workbench while transferring inoculum?
To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.
Why must it e flamed after making an inoculation?
We use to flame the inoculating loop after inoculation because during inoculation many bacterial cell get attached to loop which can further contaminate the inoculation of other cells so to destroy the previous sticked celled it is necessary to flame burn the loop
Why must the inoculating loop be cooled first before touching a culture?
To avoid killing the test subjects before the test can begin.
Why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
