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because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
What else can I help you with?
Why must it e flamed after making an inoculation?
We use to flame the inoculating loop after inoculation because during inoculation many bacterial cell get attached to loop which can further contaminate the inoculation of other cells so to destroy the previous sticked celled it is necessary to flame burn the loop
Why the loop must be flamed before streaking a new group of lines?
the inoculation loop must be flamed before streaking a new group of line to avoid any type of contamination. This is said to be one type of sterilization(dry heat sterilization) process called incineration.
Why loop and cap should not be laid on workbench while transferring inoculum?
To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.
Why is loop flamed before it is placed in a culture tube?
To kill any bacteria on it.
How will you know when you have flamed the inoculating loop or needle long enough?
You will have flamed the inoculating loop or needle long enough when the entire wire or metal is glowing red-hot. This sterilizes the tool, ensuring that no contaminants are introduced into your sample.
Why does the quadrant steak technique able to give single colonies?
Isolation streaking yields isolated colonies by dilution. When the first zone is complete, the loop is flamed and cooled, and a small number of bacteria are dragged out of zone one to complete zone two. The loop is then flamed and cooled again, and a smaller number of bacteria are dragged out of zone two to complete zone three. The loop is flamed and cooled again, and a very small number of bacteria are pulled from zone three to complete zone four.
What is the size and length of inoculation loop?
Inoculation loops can be purchased in different sizes, either in metal for flame sterilisation or in plastic for use with pathogens which are disposed of after a single use. Typically they range from 0.25 - 0.5cm internal diameter.
When getting inoculums from a slant why is it necessary to touch a sterile part of the agar with the loop before touching the bacterial growth?
It is a way to check that the loop isn't hot since you flamed it just before. I have seen some actually sizzle.
Why should the mouth of the tubes be flamed before and after inoculation?
Flaming the mouth of the tubes before and after inoculation serves to sterilize the opening and reduce the risk of contamination. This practice creates a heat barrier that helps prevent airborne microorganisms from entering the tube during the inoculation process. Additionally, it ensures that any potential contaminants on the tube's surface are eliminated, promoting the integrity of the microbial culture. Overall, this step is crucial for maintaining aseptic technique in microbiological work.
Would a false positive from the reaction between the inoculation loop and hydrogen peroxide be caused by poor specificity or poor sensitivity of the test system?
no
Why is it necessary to flame the loop between cultures?
because when we inoculate the culture with wire loop,some organism may be sticked to it which may be harmful or can cause trouble.so to destroy the remaining organisms wire loop is flamed again.
Why flaming the loop when streaking for isolation?
Flaming the loop when streaking for isolation helps to sterilize the loop by burning off any remaining bacteria from previous streaking or inoculation. This reduces the chances of cross-contamination and ensures that only the desired bacteria are being streaked onto the plate.
