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Isolation streaking yields isolated colonies by dilution. When the first zone is complete, the loop is flamed and cooled, and a small number of bacteria are dragged out of zone one to complete zone two. The loop is then flamed and cooled again, and a smaller number of bacteria are dragged out of zone two to complete zone three. The loop is flamed and cooled again, and a very small number of bacteria are pulled from zone three to complete zone four.

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