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an innoculated loop shouln't be hot. it should be cooled before contact with the organism by touching it off the edge of the agar or dipped into the top of the broth. if its hot it will kill the organism!

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16y ago

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Why should you let the inoculating loop cool first before and after using it?

The inoculating tube is cooled before use so that the organism is not killed by extensive heat.


Why cooling the inoculating instrument prior to obtaining the inoculum?

The purpose of heating the inoculating materials before and after using them is for sterilization. They must be sterilized before to kill any bacteria already on them so that they do not contaminate anything during use, and they must be sterilized after to get off the bacteria contacted from use.


How inoculating needle use?

Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.


Why should use an inoculating needle when making smears from a solid media?

You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.


Why that you should cool first the inculating loop or needle before using?

Cooling the inoculating loop or needle before using helps to prevent heat from damaging the sample being inoculated. It also prevents the inadvertent killing of the culture being transferred and helps to ensure accuracy in the transfer process. Additionally, cooling the loop or needle reduces the risk of accidental burns to the user.


How would you make sure a inoculating loop was sterile?

To sterilize an inoculating loop, it can be flamed until it turns red hot. This process helps to kill any microorganisms that might be present. It is essential to allow the loop to cool before using it to avoid damaging the culture or injuring oneself.


What is an inoculation?

Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.


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What does it mean to inoculate an agar plate?

Inoculating an agar plate refers to transferring microorganisms onto the surface of the agar using a sterile inoculating loop. This allows the microorganisms to grow and form visible colonies that can be studied or identified.


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