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Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Wiki User
∙ 10y ago
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen.
Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop.
I'm currently taking general microbiology and my lab book hardly covers this.
A.C.
Wiki User
∙ 13y ago
Wiki User
∙ 10y agoinoculating loop- liquid media or solid media in the form of plates and slants
Inoculating needle- needles are used to inoculate deep cultures by inserting the needle straight into the center of the agar. Needles can also be useful for selecting individual colonies from cultures on agar plates
Wiki User
∙ 14y agoHello students,
Why are you copy and paste the questions here...the answers lies in your lab. book....
Your micro. prof.
Wiki User
∙ 10y agosincee
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Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What is agar stab?
culture is stabbed deeply into the agar using an inoculating needle....
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
In subculturing when do you use the inoculating loop?
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
What are the other inoculating instruments aside from needle?
Aside from the inoculating needle, an inoculation loop, is a simple tool used mainly by microbiologists to retrieve an inoculum from a culture of microorganisms. Its tip is a wire made of platinum or nichrome, the latter being inferior but less expensive. The wire forms a small loop with a diameter of about 5 mm. This loop is handy for taking an inoculum from a liquid by using the phenomenon of surface tension. It is also called a smear loop, inoculation wand or microstreaker, The loop is used to cultivate microbes in Agar jelly and to use the streak maneuver to transfer microbes. The inoculation loop is always sterilized in a flame until it becomes red hot before and after each use. By doing this, the same tool can be reused in different experiments without fear of cross-contamination. After flame sterilization, the loop must be cooled so that the next cells to touch the loop don't instantly die.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What is an inoculating needle used for?
An inoculating needle is used in a microbiology lab to collect microorganisms from a culture and move them to a petri dish.
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What is agar stab?
culture is stabbed deeply into the agar using an inoculating needle....
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
Why must a straight inoculating needle be used when inoculating an agar deep tube?
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
Inoculating an agar deep tube?
inoculation needle
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
Why do you use an inoculating loop to transfer fromt he culture plates to the culture tubes?
You can transfer more because of the adhesive interactions of the water create that drop in the loop and you can spread it more easily. Also a needle could pierce the gel you transfer to because of its pointy end. The loop on the other hand distributes its force over a greater area so it does not damage the gel. This is a good answer if going from the tube to the plate, but backwards for the actual question. You use a loop to pick up a well isolated colony from your streak plate to introduce into the culture. You also can use a needle, but you won't get as many cells. However, in my work I just use sterilized toothpicks.
In subculturing when do you use the inoculating loop?
Se utiliza una vez el asa de siembra o la aguja se encuentra visiblemente roja, de manera que mueran todos los organismos. De esta manera poder esparcir a su vez la muestra en la laminilla.Translation: It is used once the loop or needle is visibly red, so that all organisms die. Thus turn to spread the sample on the slide.
What is the most commonly used instrument used for transferring cultures from a broth?
inoculating loop and needle.
