0
What is the purpose of inoculating 2 orgaisms when testing for motility?
Wiki User
∙ 9y agoWant this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What is the purpose of hydrolysis test?
There are different uses of it as it may be used to confirm sucrose by hydrolysing and than testing for reducing sugar .
What are lipid tests for?
The purpose of blood lipid testing is to determine whether abnormally high or low concentrations of a specific lipid are present.
Microbiology - what is SIM test?
Its a test where a semisolid agar called Sulfide-Indole-Motility medium (or SIM medium) is inoculated with a bacteria to test for hydrogen Sulfide, Indole, and Motility of the organism. The medium is inoculated by a swab and stab type method (rub some bacteria on the surface of the medium and stab a straight hole through the medium using a straight wire with the bacteria on it). Incubate the bacteria for about 24 hours and then begin testing.... If hydrogen sulfide is present, it will react with the sodium thiosulfate in the medium and the indicator, ferric ammonium citrate, to produce ferrous sulfide which falls out of solution as a blackish precipitate. The presence of hydrogen sulfide typically means that the bacteria produces the enzyme cysteine desulfanase which breaks up the cysteine in the medium into, among other components, hydrogen sulfide. The Indole portion of the test is performed by adding Kovac's reagent to the inoculated medium. The Kovac's reagent reacts with the indole(if indole is present) to produce a pinkish-red or redish-purple ring around the top of the test tube. If indole isn't present, there will be no color change. The presence of indole means that the bacteria produces tryptophanase, an enzyme which breaks down tryptophan into smaller components, one of which being indole. The Motility aspect of the test is done by checking the medium for turbidity, or "fuzziness". If the medium has become fairly turbid throughout the medium, then the bacteria is motile. If the medium is clear and the only turbid appearance is in the stab line, then the bacteria is non-motile. Unfortunately, the motility aspect of this test typically gives false negative results. Sometimes the temperature that the bacteria was incubated at wasn't optimum for the species, sometimes the bacteria only have weak motility, sometimes the bacteria's flagella can get damaged which would impair motility, etc... The point is, this test is good if you want to know whether or not the bacteria you're testing produces tryptophanase or cysteine desulfanase. The motility aspect of the test is suspect to question, at least if the test result was negative for motility(a large amount of turbidity in the medium is a definite sign of motility and is hard to refute though).
What is the purpose of newborn reflexes?
Testing for reflexes is an important part of the neurological physical examination in order to detect abnormalities in the central or peripheral nervous system.
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What is the purpose of controlling the environment when testing a hypothesis?
The purpose of controlling the environment when testing a hypothesis is ultimately to get a reliable result to the study.
What ingredient or step could be eliminated in the SIM medium were used strictly for testing motility among sulfur reducers?
You could first verify that you have sulfur reducers in your culture by inoculating the SIM medium and checking for the formation of the black FeS precipitate. Then, you could re-make the SIM medium excluding the ferric ammonium citrate (Fe source) and re-inoculate. There will be no black precipitate formation and you could see growth along the stab line and possibly emanating from it.
What is the purpose of presurgical testing?
Presurgical testing psychologically and physically prepares a patient for surgery.
What is the purpose of asbestos testing?
I don't know what asbestos testing is. Can somebody tell me more about it?
Which of the following statements BEST describes one of the seven key principles of software testing?
the purpose of testing
What is the purpose of testing shapes in a wind tunnel?
the purpose is they can find the best shape to go with the wind
What is the purpose of vibration testing?
The purpose of vibration testing is to ensure the mechanics or construction of something is working properly by literally shaking or vibrating the device. This can screen for mechanical, electrical or engineering flaws.
What states have mandatory HPV testing?
No states have mandatory HPV testing. Perhaps you wanted to ask about HPV vaccination. There would be no purpose or benefit to HPV testing at this time.
What is the purpose of a function generator?
A function generator creates electrical waveforms. The purpose is for developing and testing electric equipment.
What is the purpose of water testing?
To make sure your water isn't poisonous.
What is the purpose of shock testing?
Shock testing is an exhaustive means of testing a new product before it is released. It is caused shock testing because it puts the product to its limit and in extreme circumstances to check for potential shortcomings.
