0
You do a streak plate in order to get isolated colonies. If you inoculate into a slant, you have less surface area to work and less chance of getting isolated colonies. In broth, you'll definitely get growth but you won't know WHAT is growing. You go back into each quadrant (a little) with your loop in order to "dilute" the bacteria and get colonies. Quadrant 1 is pretty think (like a smear on the plate) but by the time you get to Quadrants 3 and 4, you should see more defined colonies and not just a film of bacteria.
Wiki User
∙ 10y ago
Wiki User
∙ 13y agoA streak plate is used in the isolation of a bacterium from a sample. Once isolated using the streak plate, the bacterium may be identified through sequencing of its 16S gene or through various biochemical assays. So the streak plate is only used to get single colonies to identify the bacterium...
Wiki User
∙ 13y agoYou can identify it and you can use antibiotic discs to find out what antibiotics it is sensitive to.
Wiki User
∙ 13y agoBy using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
Wiki User
∙ 13y agoTo separate out the cells so that they produce individual colonies.
Wiki User
∙ 13y agocross streak method
Add your answer:
Earn +20 pts
When would a microbiologist want to use the streak-to-grow method.?
Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.
Why do you use streak plates?
If you are talking about testing minerals, the streak plate causes powder from the mineral to come of and you rub your finger on the plate after, and see what color the dust the mineral makes is.
How could you identify a potential contaminant on a streak plate?
To clean a streak plate I use coke classic. In the red can, the diet version does not work as well. I believe it is the acid that takes away the powdered rock debris. It makes them quite white again. Mr clean magic eraser also takes away some of the heavy debris...but does not get them clean enough.
What advantage does the streak plate method have over the pour plate method?
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
What is an inoculating medium?
A media which is used to inoculate the bacterium for the culture is a inoculation media. Based on the bacteria, one can use LB media or nutrient broth or any other special media for this. The goal is to let the bacterium grow well from the inoculation culture or colony.
List two methods for obtaining isolated colonies.?
Use a "streak plate" or a "spread plate". Mediums that allow some organisms to grow and not others can also be used. These types of mediums are called Selective medium.
How is a minterals streak determinded?
Streak color is determined by scraping the mineral across a a streak plate, (which is made of unglazed porcelain), and then observing the color of the streak, which is left on the plate. Note that some minerals do not leave a streak, as they are too hard. Thus, it is important to learn other identification methods, to use in conjunction with streak color, in order to identify minerals.
How do you know a mineral's streak?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
What does a mineral and streak tell you and how do you test for it?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
What does a mineral's streak tell and how do you test for it?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
If you were given a tube with 3 different bacterial species in it what is the first thing you would do to separate the three?
The best thing to do is get a nutrient agar plate and spread the bacteria using a streak-plate method of isolation to grow the different colonies individually. (And you could do a second streak-plate from each type of colony you see just to make sure that your colonies each contain only one type of bacteria.) From there you can identify and grow each pure culture. (Also, you could use selective medias.)
How does the contribution of growth on a pour plate different from that on a steak plate?
Colonies growing on a pour plate have slightly less avalible oxygen and are confined by the gel matrix so they tend to grow smaller than those on a pour plate. Streak plates are use to isolate single colonies, pour plates are used to enumerate batceria.
What does mineral's streaks tell you and how do you test for it?
The streak of a mineral can distinguish between two samples that have the same color. The streak is often a different color. To test streak, use a streak plate. This is a piece of unglazed porcelain, like the back side of a tile.
What is the significance of using a 'dry and hard' agar plate in streak plating?
Why is it impotant to use dry and hard agar for streaking
When would a microbiologist want to use the streak-to-grow method.?
Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.
When would a microbiologist want to use the streak-to-grow method?
Plate streaking is often done to isolate a colony of bacteria. For instance, if a broth was grown with 2 or 3 different types of bacteria, it could be streaked out in a "3-phase streak." In a 3-phase streak the initial streak takes up a very small area (we draw a T on the back of the plate, this streak goes in the section above the T). The loop is flamed to kill off any bacteria still on it, then a couple streaks are made out of the original. This grabs some of the bacteria from that concentrated streak and spreads them out. This is repeated once more until the final streaks are less and less concentrated bacteria. When we incubate the plate we'll find lots of growth where the original streak was and less and less as we follow the path of the 3-phases. What you're looking for now is a single colony, off by itself. This can then be scraped off and plated on a separate plate and considered a "pure colony." Another usual time to use streak-to-grow bacteria is when you want to know the quantity or concentration of bacteria. You take maybe 0.1mL of solution and plate it, then count the number of colonies that form. Say you have 56 colonies, now you can say: 56 cfu (colony forming units) -------- 0.1 mL or 560 cfu/mL This is usual when testing to see whether a sterilization technique worked, or if a product is within the regulated levels of bacteria to be released the public, etc etc.
Why do you use streak plates?
If you are talking about testing minerals, the streak plate causes powder from the mineral to come of and you rub your finger on the plate after, and see what color the dust the mineral makes is.
