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To clean a streak plate I use coke classic. In the red can, the diet version does not work as well. I believe it is the acid that takes away the powdered rock debris. It makes them quite white again. Mr clean magic eraser also takes away some of the heavy debris...but does not get them clean enough.

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12y ago
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14y ago

In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri dish plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony

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15y ago

A contaminant is anything other than the organism(s) you were trying to grow. This question is a little like asking what plants are weeds and there is no 100% true answer. The purpose of a streak plate is to gain single isolated colonies. The most troublesome species to find growing on your streak plates are swarming bacteria as they spread and contaminate other colonies, pseudomonas sp. are an example. The contaminating bacteria could have come from the operative or from the room where the procedure was carried out.

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10y ago

The spread plate can become contaminated if you forget to sterilize your "hockey" stick or leave the plate open on a counter.

See the Related Question for more ways the spread plate can become contaminated.

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11y ago

More ways than you can count. Forget to sterilize your 'hockey' stick, have a contaminated culture to begin with, leave the plate open on the bench, sneeze while spreading, have a air conditioner turn over while spreading, have a sloppy bench mate, etc.....

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13y ago

It would most likely be growing on an area over which you didn't streak.

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10y ago

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Q: How could you identify a potential contaminant on a streak plate?
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