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dNTP's are the building blocks for new strands.

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dNTPs (deoxynucleoside triphosphates) are the building blocks used by DNA polymerase to synthesize new DNA strands during PCR. They provide the necessary bases (A, T, C, G) for complementary base pairing with the template DNA strand. This results in the amplification of the target DNA sequence.

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used in PCR. They're building blocks of DNA which includes bases A C G T

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The dNTPs are building blocks of new DNA strand.

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Q: What is the role of dNTP in PCR?
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PCR (polymerase chain reaction) is a molecular biology technique used to amplify a specific segment of DNA. There are various types of PCR, including quantitative PCR (qPCR) for quantification of DNA, reverse transcription PCR (RT-PCR) to amplify RNA, nested PCR for increased specificity, and digital PCR for absolute quantification of nucleic acids.

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An essential cofactor for the DNA polymerase in PCR is Magnesium chloride. Its concentration must be optimized for every primer:template system. Many components of the reaction bind magnesium ion, including primers, template, PCR products and dNTPs. The main 1:1 binding agent for magnesium ion is the high concentration of dNTPs in the reaction. Because it is necessary for free magnesium ion to serve as an enzyme cofactor in PCR, the total magnesium ion concentration must exceed the total dNTP concentration. Typically, to start the optimization process, 1.5 mM magnesium chloride is added to PCR in the presence of 0.8 mM total dNTPs. This leaves about 0.7 mM free magnesium for the DNA polymerase. In general, magnesium ion should be varied in a concentration series from 1.5-4.0 mM in 0.5 mM steps.I just read somewhere that some PCR reagents require free Mg2+

Role of magnesium chloride in PCR?

Magnesium chloride is a crucial component in the polymerase chain reaction (PCR) as it is required for the activity of the DNA polymerase enzyme. Magnesium ions help stabilize the DNA template-primer complex and are essential for the enzymatic activity of the DNA polymerase, allowing for successful DNA amplification during PCR. The optimal concentration of magnesium chloride can vary depending on the specific DNA polymerase being used and the PCR conditions.

What is pcr primer design ROLE?

PCR primer design is crucial for the success of a PCR reaction. Primers must be complementary to the target DNA sequence to initiate DNA amplification. Factors such as primer length, GC content, melting temperature, and primer-dimer formation should be considered during primer design to ensure specific and efficient amplification.

What is the defference between Real-time PCR and reverse transcriptase PCR?

Real-time PCR is a technique used for quantifying DNA in real-time during the PCR process, while reverse transcriptase PCR (RT-PCR) is used to detect RNA by first converting it to complementary DNA (cDNA) using reverse transcriptase enzyme before proceeding with PCR amplification. Real-time PCR allows for monitoring the amplification process as it occurs, while RT-PCR is specifically used for analyzing RNA levels.

Why do you use a negative control in PCR?

A negative control is used in PCR to ensure that there is no contamination in the reaction, which could lead to false positive results. It contains all the PCR components except the template DNA, so any amplification detected in the negative control would indicate contamination.

What is the function of EDTA in PCR?

EDTA is typically added to PCR reactions to chelate divalent cations present in the reaction mixture, such as magnesium ions, which can inhibit the activity of certain enzymes like DNA polymerase. By sequestering these ions, EDTA helps to maintain enzyme activity and improve the efficiency of DNA amplification during PCR.