This question gave me cancer.
lyses cells
Its purpose is to isolate DNA from a protein mixture.
Urea is a chaotropic agent, and its role is obviously denature proteins and DNA, and promote more stability to the system, breaking the hydrogen ligations between DNA and water and making the intramolecular ones more stronger.
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
STE stabilize the lysosom's membrane stop the proteinase liberation
It plays a role.
roll of Na CL in DNA extraction
The actual role of phenol chloroform isoamyl alcohol in a plasmid DNA extraction is to purify the DNA. The alcohol will act in part as a detergent.
lyses cells
Its purpose is to isolate DNA from a protein mixture.
Urea is a chaotropic agent, and its role is obviously denature proteins and DNA, and promote more stability to the system, breaking the hydrogen ligations between DNA and water and making the intramolecular ones more stronger.
To give the solution buffering capacity.
stabilization of phenol against oxidation
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
ammonium acetae use to percipitate DNA from water.
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
sodiom acetat reaction with membrane protein and cause that persipitate and help to dna isolation