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To make DNA probes
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What radioactive isotope did hershey and chase use to tag phage DNA?
phosphorus - 32P
Why do plants need phosphorus?
Inorganic phosphorus in the form of the phosphate PO43- plays a major role in biological molecules. Plants need phosphate from the soil to make their DNA.
What percentage of DNA will be radioactive after 3 DNA replication cycles?
3%
IF DNA is labeled radioactive and allowed to replicate how much radioactivity in newly synthesized DNA?
WHAT IS DNA MADE FROM>
What kind of molecule must be labeled to make radioactively labeled DNA?
Radioactive nucleotide
Which molecule for which phosphorus-32 is used as a radioactive marker?
Dna
What happens when T2 phages are grown in the presence of radioactive phosphorus?
The bacterial proteins will become radioactive
What reaction is used to radioactivley label DNA?
DNA is labeled a radioactive phosphorus because when Alfred looked at the examples 32p always pelleted with bacteria but the new Phage made by these infected bacteria contained Radioactive 32p which is why its labeled Radioactive phosphorus.
What radioactive isotope did hershey and chase use to tag phage DNA?
phosphorus - 32P
Why do plants need phosphorus?
Inorganic phosphorus in the form of the phosphate PO43- plays a major role in biological molecules. Plants need phosphate from the soil to make their DNA.
When was Integrated DNA Technologies created?
Integrated DNA Technologies was created in 1987.
Would it be hard for plants to make DNA with a shortage of phosphorus in the soil?
Yes Phosphorus is essential in the production of DNA for plants.
What must be done to ensure DNA in a laboratory is radioactive?
Incorporating radioactive nucleotides must be done to ensure that DNA made in a laboratory is radioactive.
How did Hershey and chase show that DNA was the molecule that carried the genetic code?
Hershey (you know, Alfred Hershy) and Chase (you know again, Martha Chase) did some experiments with Bacteriophages (you know, viruses that affect Bacteria). What they did in the experiment was that, they grew some of these Bacteriophages on a medium which contained radioactive Sulphus(S)(remember, radioactivity can be detected easily). Then, they also took some other Bacteriophages. But, they were grown on radioactive Phosphorur(P)..... (Don't 4get(that's forget)), DNA also has Phosphorus in it (DNA = Nitrogen bases + Pentose sugar + Phosphorus). Now, back to the experiment ------ The Bacteriophages that were grown on radioactive Phosphorus contained radioactive DNA. But they didn't have any radioactive protein coat. (remember viruses have a protein coat covering their DNA?) Then, bacteriophages (please call them B from now, but not in the exams), which were grown in S (radioactive sulphur, remember?), had a radioactive protein coat (protien coats are made up of sulphur and some other elements), but no radioactive DNA........ That was the first part....................... Now, the next one.......................... Radioactive B's, from both P and S were allowed to attach to E.coli (Esterichia coli) bacteria as an attack (or infection). After infection, The protein coats were removed by agitating (stirring real fast) in a blender (they're still not removed). Then, these virus particles (protein coats) wer removed by centrifugation (using centrifugal machine). Then, the third big step....................................… The observation part....................................… It was observed that, some bacteria were radioactive and many were not. Also, it was observed that, bacteria which were attacked by B (Bacteriophage) having radioactive DNA were the ones which were radioactive..... Also that, bacteria which were attacked by B having S weren't radioactive...... So, it lead to the point that proteins didn't pass into bacteria, but it was the DNA that did...... So, DNA was concluded to be the genetic material...... Viruses were chosen because they only had genetic meterial & coat This is from yahoo anwsers btw
What is meant by the term Idt DNA?
The term Idt DNA stands for Integrated DNA technologies. Integrated DNA Technologies develops and manufactures products for life sciences researchers.
What organic compound contains phosphorus?
DNA
What are phosphorus's used for?
There are many uses of phosphorus such as: 1. Phosphates are used to make special glass which is used for sodium lamps 2. Plants needs phosphorus for nutrition. Thus, it is added in fertilizers; 3. Radioactive isotopes of phosphorus are used as radioactive tracers; 4. China ware are made up of calcium phosphate 5. Matchstick strikers are made up of phosphorus 6. Incendiary Bombs, Tracer Ammunition, and Smoke Screen are made up of white phosphorus 7. One compound of phosphorus called tributylphosphate is used for extracting uranium 8. DNA and RNA makes use of phosphorus 9. Phosphorus is also used in manufacturing steel 10. It is used as detergent (Sodium tripolyphosphate) 11. Used for manufacturing fertilizers, toothpaste, food additives and pesticides (0ther compounds of Phosphorus)
