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Good question! The basic idea is to cause particles of varying sizes to move through a gel made up of some physical 'mesh'. Common biological gels are made of Agarose proteins (for separating nucleic acids) or Acrylamide/bisAcrylamide 'mesh' for separating proteins. Smaller molecules can move through the network faster, and so will move farther in a particular time period (minute, half-hour, hour, whatever). So, if you run a gel for 30 minutes or so, you will have a distribution of sizes, with the smallest pieces out in front, and the larger ones progressively further behind. Often you will have these molecules moving in 'bands', which are clumps of lots of a molecule which are the same size. Usually, you get the proteins or nucleic acids to move by running a current across the gel, as these molecules have a slight electrical charge. If you also run a set of comparable molecules of known sizes (usually called a 'size standard') then you have something to compare your results to so you can tell what size a particular band is. You can have a 'separating gel' game yourself, if you want: set up an obstacle course (with tunnels and other barriers of various kinds), get about 30-50 people, with kids of all ages and adults of all ages, and have them all start at the same time. Then, when the first person gets about 2/3 of the way through, blow a whistle and have them all stop where they are. Then see how they're arranged - take a photo so you can have a visual record of your experiment!
Wiki User
∙ 15y ago
Wiki User
∙ 9y agoSeparating gel allows the separation of protein molecules according to their molecular weight by sieving effect of pores in the gel(percentage). The pH of separating or resolving gel is 8.8, whereas stacking gel (upper gel that squeezes protein as a thin layer) made of pH6.8.
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What is an agarose gel and what is it used for?
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
What is the appearance and texture of the vitreous humor?
The vitreous humor is a jelly like liquid that fills most of the eye (from the lens back). As we age it changes from a gel to a liquid and gradually shrinks separating from the retina. See related link for more details.
What is an example of a gel?
example of gel is agarose gel,
How does electrophoresis work?
Electrophoresis for nucleic acids (RNA and DNA) works by separating segments by their size. This is possible because RNA and DNA are negatively charged, so will move towards the positive charge applied to one end of the gel. The different segments separate because small fragments of RNA or DNA are able to move more quickly through the gel than larger fragments.
What could be done to resolve the fragments?
answer from another site You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating). Or try polyacrilamide. It should solve those bands even better.
What is the function of the gel?
Separating gel allows the separation of protein molecules according to their molecular weight by sieving effect of pores in the gel(percentage). The pH of separating or resolving gel is 8.8, whereas stacking gel (upper gel that squeezes protein as a thin layer) made of pH6.8.
What materials do you need to do a gel electrophoresis?
depends on what your separating??
What are the name of separating techniques involving electric charge on molecules?
Gel electrophoresis.
What is an agarose gel and what is it used for?
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
What is the principle for agarose gel electrophoresis?
it is a technique that separated dna according to its size.
What is the principle used in separation of mixtures?
separating an insoluble solid from a liquid: decantation, filtration separating a dissolved solid (solute) from a solution: evaporation, crystallization separating the solute and solvent from a solution: simple distillation separating a mixture of two miscible liquids: fractional distillation
What is the purpose of the gel inside of certain tubes?
For venous blood specimens, if it is test tubes used in blood collection that you are referring to, the gel is a serum separator. When the sample is centrifuged, the red cells will spin to the bottom, plasma to the top, gel separating the blood components.
How can you fix your dreadlocks to look good?
just do the front of your hair by separating each lock and using gel and claps do the ones in the front
What could be done to resolve two fragments that are nearly the same base-pair length and did not appear on the electrophoresis gel?
You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating).
What is the explanation for the principle operation of wave analyzer?
An apparatus that assesses a complex mixture of wave forms by separating out their component frequencies and displaying their distribution.
Which chemical principle comes into play during an increase in viscosity of a carbomer gel?
stop cheating at the reveal loreal game :D
Which of the following best describes how scientists use gel electrophoresis?
Gel Electrophoresis is the technique for sorting DNA molecules based on their number of base pairs by running a current through the gel. The sample travels opposite of the direction of the electrical charge since DNA is negatively charged. Using a certain primer you can identify the BP of the sample.
