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What is the sequence of the template strand if a nontenplate strand has the sequence 5'ATGGGCGC3'?
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How does the nucleotides on one strand of DNA provide a template or guide for the sequence of subunits on the other strand?
They bond, and make a replication of itself.
Is TATA box is present on plus strand or minus strand?
yes, the TATA box (and broader idea, the promoter) is sequence read on the sense (non-template) strand for convention.
What is important about the DNA template strand?
The template and non-template strands of DNA are complementary.This means that if a T (thymine)occurs on one strand, there must be an A (adenine) in that position on the other strand, and that C (cytosine) is always opposite G (guanine), following the rules of complementary base pairing.There are other names for the two strands, but Googling them shows there is a lot of confusion out there! The terms "template strand" and "non-template stand" seem to be the only ones that everyone uses consistently. The template strand is the strand along which messenger RNA is synthesized, and has, of course, a base sequence complementary to that of the RNA.The term "gene" is often applied to the non-template strand, the argument being that the non-template DNA strand and the mRNA have the same base sequence (except that where DNA has T, RNA has U, uracil).In transcription, RNAP uses template strand to make a copy of mRNA. Complementary to template strand is the coding strand, which sequence is identical to mRNA sequence except for the substitution of U for T. Although the coding strand is not used as a template for common transcription events, it is called coding because its sequence is used as a copy in mRNA sequence. For the case of "sense", terminologically template strand is called antisense, and coding strand is called the sense strand.Template/non-coding/antisenseNon-template/coding/senseMany people confuse complementary sequences with palindromic sequence which you can find in restriction system recognition sequences. Although the template strand yields a sense (functional) sequence in mRNA and thus a properly-folded protein, the complementary strand of it, non-template strand upon being transcribed yields a totally different and non-functional protein. However in terms of transcription of palindrome, both strands yield the same mRNA sequence, thus the same protein.Coding strand of a particular gene can be on one of either two strands of DNA, and thus this applies to the opposite strand of the said strand for the non-coding strand. The direction of transcription on a double-stranded DNA depends on whether the upper or lower strand is being transcribed. Therefore on a linearised genome, transcription occurs to the left for certain genes and to the right for the remaining genes.
What is primer in pcr?
A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.
If the base sequence of template strand is GCCATTAC what would the base sequence of the mRNA?
The mRNA will have codons AUG-CCA-GUA-GGC-CAC
What is the coding DNA and mrna strand for the template strand 3' a-g-g-t-t-c-a-t 5'?
The top strand, which is drawn 5' to 3' and which contains the promoter sequences in the conventionally written orientation (such as the TATA box) and which has the same sequence as the new RNA (except for U instead of T) is the plus strand or the sense strand or the non template strand or the coding strand. The bottom 3' to 5' strand is the minus, or template, or antisense strand. Your sequence therefore is the coding strand, but the RNA is transcribed off of the non-coding, template, or antisense strand.
How does the nucleotides on one strand of DNA provide a template or guide for the sequence of subunits on the other strand?
They bond, and make a replication of itself.
What is the amino acid sequence after the insertion occurred?
The template strand is mutated to 3'-TACTTGTCCAATATC-5'.
What does it mean to say DNA polymerase reads a template strand to make the complementary strand?
During DNA replication, the enzyme DNA polymerase catalyses the formation of new strands of DNA, using the old strands as models. DNA has a double-helix structure, with two strands forming each helix. Each strand is made up of DNA nucleotides, with the genetic information encoded in the sequence of different nucleotides (different nucleotides are distinguished by molecules called 'bases' attached to them, so the sequence of nucleotides is known as the 'base sequence'). The base sequence of one strand is complementary to that of its' neighbour - the base A binds with T, and C with G, so if one strand had the sequence ATTACA, the base sequence of the complementary strand would be TAATGT. When DNA polymerase creates a new DNA strand, it does so by matching nucleotides to the base sequence of one of the strands - the template strand. New nucleotides are brought in, which match the template in a complementary fashion (ie. A-T, C-G), and join to become one new strand. This new strand is complementary to the template.
Is TATA box is present on plus strand or minus strand?
yes, the TATA box (and broader idea, the promoter) is sequence read on the sense (non-template) strand for convention.
What is important about the DNA template strand?
The template and non-template strands of DNA are complementary.This means that if a T (thymine)occurs on one strand, there must be an A (adenine) in that position on the other strand, and that C (cytosine) is always opposite G (guanine), following the rules of complementary base pairing.There are other names for the two strands, but Googling them shows there is a lot of confusion out there! The terms "template strand" and "non-template stand" seem to be the only ones that everyone uses consistently. The template strand is the strand along which messenger RNA is synthesized, and has, of course, a base sequence complementary to that of the RNA.The term "gene" is often applied to the non-template strand, the argument being that the non-template DNA strand and the mRNA have the same base sequence (except that where DNA has T, RNA has U, uracil).In transcription, RNAP uses template strand to make a copy of mRNA. Complementary to template strand is the coding strand, which sequence is identical to mRNA sequence except for the substitution of U for T. Although the coding strand is not used as a template for common transcription events, it is called coding because its sequence is used as a copy in mRNA sequence. For the case of "sense", terminologically template strand is called antisense, and coding strand is called the sense strand.Template/non-coding/antisenseNon-template/coding/senseMany people confuse complementary sequences with palindromic sequence which you can find in restriction system recognition sequences. Although the template strand yields a sense (functional) sequence in mRNA and thus a properly-folded protein, the complementary strand of it, non-template strand upon being transcribed yields a totally different and non-functional protein. However in terms of transcription of palindrome, both strands yield the same mRNA sequence, thus the same protein.Coding strand of a particular gene can be on one of either two strands of DNA, and thus this applies to the opposite strand of the said strand for the non-coding strand. The direction of transcription on a double-stranded DNA depends on whether the upper or lower strand is being transcribed. Therefore on a linearised genome, transcription occurs to the left for certain genes and to the right for the remaining genes.
What is primer in pcr?
A primer (oligonucleotide of a specific sequence) is required for Taq polymerase to extend the template strand by adding complementary nucleotides. The function of the primer is to anneal to the template strand at a very specific site and facilitate the initiation of strand elongation mediated by Taqploymerase.
What is the nucleotide sequence of the complementary strand of the dna molecule t t c g a a t t g c?
The sequence of nucleotides of the complementary strand will be the nucleotides which bind to the nucleotides of the template. In DNA, adenine binds to thymine and cytosine binds to guanine. The complementary strand will therefore have an adenine where the template strand has a thymine, a guanine where the template has a cytosine, etc. For example: If the template strand is ATG-GGC-CTA-GCT Then the complementary strand would be TAC-CCG-GAT-CGA
If the base sequence of template strand is GCCATTAC what would the base sequence of the mRNA?
The mRNA will have codons AUG-CCA-GUA-GGC-CAC
How does DNA template dictate the sequence of the RNA produced?
During transcription the DNA double helix is separated into two individual strands. Each strand may serve as a template for RNA polymerase, which travels along the DNA structure in a 3' to 5' direction. As it progresses down the strand, RNA polymerase synthesizes a pre-messenger RNA strand that is complementary to the sequence on the DNA template. For example if the DNA sequence on the template was 5' ATACA 3', then the pre mRNA sequence synthesized would be 3' UAUGU 5'. (Remember, RNA synthesis utilizes the nucleotide uracil instead of thyamine).
What is the non template strand?
The difference between the coding strand and the template strand is the coding strand is the strand which contains the coding genes, i.e. the one in which the RNA polymerase reads and transcribes into mRNA. It must have the promoter sequence in the correct orientation for transcription, as follows:5`-TATAATGCGCGCGCGCGCGCGCGC-3`3`-ATATTACGCGCGCGCGCGCGCGCG-5`In this sequence, the top strand is the coding strand, because it contains the promoter (TATAAT) in the correct orientation.However, when transcribed, the mRNA will be as follows:5`-GCGCGCGCGCGCGCGCGCGC-3`This is because the polymerase transcribes from the template strand, on the opposide side to the coding strand, to make it in the same orientation as the coding strand.I hope I have explained it enough for people to understand, however if I haven't please read this article I found which explains it thoroughly:http://www.bio.net/bionet/mm/bioforum/1994-May/008821.html
The direction of synthesis of an RNA transcript is?
transcription:"the first step in protein synthesis, a sequence of nucleotide bases becomes exposed in an unwound region of a DNA strand. That sequence acts as a template upon which a single strand of RNA - a transcript - is synthesized from free nucleotides."The synthesis of an RNA molecule from the DNA template strand is called transcription.
