Long answer, it depends on your objective lens your ocular lens (magnification, quality, medium, etc).
Simple answer is 0mm, nm, whatever. What is in "Sharp" focus is only a single 2-dimensional plane having no 3rd dimension (thickness). Therefore its "thickness" is infinitely small.
Given however that the human eye can only distinguish objects larger than 0.2mm, the 2-dimensionality is irrelevant, and the answer you are looking for lies in the details I mentioned above, objective magnification, ocular magnification, whether you are using oil immersion or not, whether you are using a blue filter or not.
Of course all of this is also assuming you are talking about a light microscope.
The depth of field is the part of a specimen that is in sharp focus; the depth of field decreases as the NA increases. The depth of focus on the other hand is the magnified image in focus on the film plane; depth of focus decreases as magnification increases. To learn more about microscopes and its uses visit the website in the link below.
The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.
exfoliative cytology is a technique in which cells are scraped from the tissue and examined under a microscope. To exfoliate means to remove a specimen flakes or scales.
This can vary though most typically it a 2, 3 or 4 layer structure incorporating an aluminum or metallised aluminum layer to provide the key barrier performance. It will have an inner layer of polyethylene that is readily heat sealable and also provides protection to the key aluminum layer and robustness to the overall structure. This layer can be clear or pigmented and there is a range of types of polyethylene that each bring other attributes such as higher oil/grease resistance, stronger heat seals, sealed with less energy or faster throughput. The top layer will typically be a transparent layer of Polyester or Nylon to give, high puncture resistance, and also add to barrier to gases. A further layer of this can often be incorporated between the foil and the polyethylene layer to protect the foil from sharp, granular ingredients and to boost tear resistance still further if required.
No you cannot compact sharp sand
For the adjustment of focus, you would have to use the Fine-adjustment knob located on the left and right sides of the microscope arm.
There are two knobs. The "coarse focus" knob changes the focal length very quickly, so you use that to get a close to sharp image. Then, you use the "fine focus" knob to perfect the image. Both knobs do the same thing, just at different rates. Resource: http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm
The depth of field is the part of a specimen that is in sharp focus; the depth of field decreases as the NA increases. The depth of focus on the other hand is the magnified image in focus on the film plane; depth of focus decreases as magnification increases. To learn more about microscopes and its uses visit the website in the link below.
use the fine focus knob until we get sharp image
To bring a sharp focus.
lenses: set up or arranged so that when one lens is changed for another, there is no change in focus. * Applied usually to turret mounted lenses e.g. on microscope; also in telescopes: ~ eyepieces: changing one for another does not require refocusing. * When the distance from the object to the rear principal plane of each lens is equal, they are said to be parfocal.
be better
because it has many compound parts to the microscope ex. body tube, ocular lens,coarse adjustment(rough), and fine adjustment (sharp)
Auto Focus
The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.
that is not a telescope that is a microscope. and most likely an electron microscope depending on what type they have the highest magnification of any other microscope.
it can do to the singurlity of a blackhole.