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Thioglycolate (thio) broth contains Sodium thioglycolate, a reducing agent that creates anaerobic conditions when it reduces molecular oxygen to water. Dyes such as resazurin or methylene blue are usually added to the broth to provide a visual indication of the presence of oxygen. Resazurin is pink when oxidized and colorless when reduced. Methylene blue is blue when oxidized and colorless when reduced. The pink band near the top of the broth results when oxygen diffuses in. Strict aerobes will grow only in the pink band, microaerophiles will grow near the bottom of the band where the concentration of oxygen is lower. The absence of pink in the rest of the tube indicates the absence of oxygen and a suitable environment for strict anaerobes. Both facultative anaerobes and aerotolerant anaerobes will grow throughout the tube; however, facultative anaerobes will grow most densely where oxygen is present. Other constituents:L-Cysteine Yeast Extract Glucose Agar(may be or not) Peptone Distilled Water. *The use of thioglycolate broth permits growth of anaerobic bacteria. In addition, growth patterns can help distinguish aerotolerance of bacteria. E.g., Clostridium sporogenes, Clostridium perfringens. PROCEDURE Step 1. Label 2 Blood Agar plates, one plate will be titled "Aerobic" and the other titled"Anaerobic". Step 2. Using your inoculating loop, streak each plate with Clostridium perfringens. Step 3. Place the anaerobic Blood Agar plate in a Bio-Bag or Gas Pac Jar. The instructor will demonstrate proper set up of the environmental chamber. Step 4. Incubate both plates at 37C for 24-48 hours. Step 5. Obtain Three Thioglycolate Broth tubes -DO NOT SHAKE THESE TUBES!. Gently label each Thioglycolate Broth with one of the assigned species being careful not to tip the tube horizontally . Step 6. Using your inoculating loop, inoculate each Thioglycolate Broth with it's assigned species. Step 7. Incubate the Thioglycolate Broth at 37C for 24-48 hours. Note: Procedure taken fromhttp://bioweb.wku.edu/courses/Biol208/Lab_Manual/208%20week%2011.pdf.Dr.Ank
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Why is thioglycolate broth stored at room temperature?
There is a decreased absoportion of oxygen by the mediium.
Explain the results in the sterilized and not-sterilized broth?
Sterilized broth is clear, meaning no bacterial growth. Non-sterilized broth is cloudy.
Why is nutrient broth better than peptone broth?
Nutrient broth contains beef extract which acts as sources of amino acids, peptides, nucleotides, organic acids, vitamins and mineralsthat peptone broth don't contain? :)
How long has broth been in our culture?
Broth has been in our culture since the beginning of time.
What is ratio between blood and broth in blood broth?
blood should be 5% by solution
Why is thioglycolate broth stored at room temperature?
There is a decreased absoportion of oxygen by the mediium.
What is thioglycolate?
Thioglycolate (thio) broth contains Sodium thioglycolate, a reducing agent that creates anaerobic conditions when it reduces molecular oxygen to water. Dyes such as resazurin or methylene blue are usually added to the broth to provide a visual indication of the presence of oxygen. Resazurin is pink when oxidized and colorless when reduced. Methylene blue is blue when oxidized and colorless when reduced. The pink band near the top of the broth results when oxygen diffuses in. Strict aerobes will grow only in the pink band, microaerophiles will grow near the bottom of the band where the concentration of oxygen is lower. The absence of pink in the rest of the tube indicates the absence of oxygen and a suitable environment for strict anaerobes. Both facultative anaerobes and aerotolerant anaerobes will grow throughout the tube; however, facultative anaerobes will grow most densely where oxygen is present. Other constituents:L-Cysteine Yeast Extract Glucose Agar(may be or not) Peptone Distilled Water. *The use of thioglycolate broth permits growth of anaerobic bacteria. In addition, growth patterns can help distinguish aerotolerance of bacteria. E.g., Clostridium sporogenes, Clostridium perfringens. PROCEDURE Step 1. Label 2 Blood Agar plates, one plate will be titled "Aerobic" and the other titled"Anaerobic". Step 2. Using your inoculating loop, streak each plate with Clostridium perfringens. Step 3. Place the anaerobic Blood Agar plate in a Bio-Bag or Gas Pac Jar. The instructor will demonstrate proper set up of the environmental chamber. Step 4. Incubate both plates at 37C for 24-48 hours. Step 5. Obtain Three Thioglycolate Broth tubes -DO NOT SHAKE THESE TUBES!. Gently label each Thioglycolate Broth with one of the assigned species being careful not to tip the tube horizontally . Step 6. Using your inoculating loop, inoculate each Thioglycolate Broth with it's assigned species. Step 7. Incubate the Thioglycolate Broth at 37C for 24-48 hours. Note: Procedure taken fromhttp://bioweb.wku.edu/courses/Biol208/Lab_Manual/208%20week%2011.pdf.Dr.Ank
Which perms do not have sodium thioglycolate?
Ammonium thioglycolate is an alternative to sodium thioglycolate in perms, as it offers a gentler solution for those with sensitivities. Thioglycolic acid is another common ingredient used in perms, but it is important to always check the product label to be sure of the ingredients.
What are the ingredients in cold cream?
PEG-4 Polyglyceryl-2 Stearate6.00ACeteareth-3AMineral Oil, high viscosityACoconut OilAIsopropyl PalmitateAAntioxidantBPropylene GlycolBUreaCCalcium Thioglycolate (trihydrate)CCalcium Hydroxide, powderDFragranceDPropylene Glycol; Diazolidinyl Urea; Methylparaben; Propylparaben
Is thioglycolate dangerous to use?
Thioglycolate can be dangerous to use if not handled properly. It is a strong reducing agent that can cause skin irritation, burns, and allergies. It is important to wear proper protective equipment, work in a well-ventilated area, and follow safety precautions when handling thioglycolate.
What is the common ingredient in a thio type relaxer and permant waving solution?
ammonium thioglycolate, I believe....
What does appearance of a blue or pink color in thioglycolate tube mean?
To indicate a presence of free residual oxygen.
What chemicals are in depilatories?
Depilatories commonly contain chemicals like calcium thioglycolate or potassium hydroxide, which work to break down the protein structure of hair, making it easier to remove from the skin. Ingredients such as mineral oil and aloe vera may also be included to help soothe and moisturize the skin.
Can chicken broth cause salmonella?
Yes, if the chicken used in the broth has salmonella, then it is possible that the broth will have it as well.
How do you use broth in a sentence?
i want chicken broth
What is soup broth made of?
Soup broth is usually made from meat or vegetable broth. The broth is made by boiling meat or vegetables in water. They are then removed from the water, which flavored by the cooking food, becomes what is called broth.
Is broth has agar?
no, broth stands for liquid and agar is used as solidifying agent so broth is not containig agar.
