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What is transcriptome?

Updated: 12/19/2022
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Q: What is transcriptome?
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What is the relationship of a transcriptome to the genetic code?

A transcriptome is the percentage of the genetic code that is turned into RNA molecules. It transcribes to less than five percent of the genome in humans.


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What has the author Xin He written?

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What are the Effects of pine oil on bacterial blight?

Genetic changes that correlate with the pine-oil disinfectant-reduced susceptibility mechanism of Staphylococcus aureus. Lamichhane-Khadka R, Riordan JT, Delgado A, Muthaiyan A, Reynolds TD, Wilkinson BJ, Gustafson JE.SourceDepartment of Biology, New Mexico State University, Las Cruces, NM 88003-8001, USA. AbstractAIMS:To identify factors associated with the Staphylococcus aureus pine-oil disinfectant-reduced-susceptibility (PD(RS)) mechanism and to describe one possible PD(RS) model.METHODS AND RESULTS:Comparative genomic sequencing (CGS) and microarray analysis were utilized to detect mutations and transcriptome alterations that occur in a S. aureus PD(RS) mutant. Mutant analysis, antimicrobial gradient plates, growth studies and 3-hydroxy-3-methylglutaryl coenzyme A synthase assays were then performed to confirm the biological consequences of the 'omics' alterations detected in a PD(RS) mutant. CGS uncovered three mutations in a PD(RS) mutant in a(n): alcohol dehydrogenase (adh), catabolite control protein A (ccpA) and an NADPH-flavin oxidoreductase (frp). These mutations lead to increased growth rates; increased transcription of an NAD-dependent D-lactate dehydrogenase gene (ddh); and increased flux through the mevalonate pathway. PD(RS) mutants demonstrated reduced susceptibility to bacitracin and farnesol, and one PD(RS) mutant displayed upregulation of bacA, a bacitracin-resistance gene. Collectively, this evidence demonstrates altered undecaprenol metabolism in PD(RS) mutants.CONCLUSIONS:The PD(RS) mechanism proposed results from increased catabolic capabilities and increased flux through the mevalonate pathway as well as altered bactoprenol physiology.SIGNIFICANCE AND IMPACT OF THE STUDY:A novel mechanism that bacteria utilize to overcome the killing effects of PD formulations is proposed that is unique from the PD(RS) mechanism of the enterobacteraciae.


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