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The Clinical Chemistry Section of a Hospital Pathology Laboratory will prpbably undertake protein electrophoresis on the blood samples from patients.
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What is protein marker and what is its use in electrophoresis?
it is a marker for specific protein identification.in electrophoresis it is used to identify particular protein based on the molecular weight.
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
Protein electrophoresis and how its carried out?
An example of protein electrophoresis is SDS-PAGE ( sodium do-decyl sulpahate-polyacrrlamide gel electrophoresis).Another example includess " isoelectric focusing".In isoelectric focusing the protein is separated on the basis of its net charge.The main principle lies on the basis of finding isoelectric point i.e. at which the net charge on the protein is zero.The protein is loaded in the gel and then it separates itself on the basis of the charge.NEgatively charged on the negative side and positively gharged on the positive side and the neutral ones in the centre.
What is cause of faster protein migration in electrophoresis?
Simply, smaller proteins migrating through the holes tunneled in the gel.
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
What is protein marker and what is its use in electrophoresis?
it is a marker for specific protein identification.in electrophoresis it is used to identify particular protein based on the molecular weight.
What has the author A J Houtsmuller written?
A. J. Houtsmuller has written: 'Agarose-gel-electrophoresis of lipoproteins' -- subject(s): Blood protein electrophoresis, Electrophoresis, Gel electrophoresis, Lipoproteins
Why agarose gel electrophoresis is not used for protein analysis?
eating
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
Difference between vertical and horizontal gel electrophoresis?
Horizantal gel electrophoresis is generally used for RNA/DNA based studies, while vertical gel electrophoresis is used for protein based studies.
What has the author Samantha Bradd written?
Samantha Bradd has written: 'Protein electrophoresis'
Is DNA smaller or bigger than a protein?
Let's put it this way, we know that electrophoresis is a test for the sizes of the fragments of DNA molecules while SDS-page is a test of the size of protein molecules. If you use electrophoresis to test the differences of protein, there will not be any bands as all the protein will travel to the end of SDS-page. Therefore, we can conclude that the pores of electrophoresis is much more larger than SDS-page. Since electrophoresis has larger pores than SDS-page, it also shows that overall DNA is larger than protein in size.
Protein electrophoresis and how its carried out?
An example of protein electrophoresis is SDS-PAGE ( sodium do-decyl sulpahate-polyacrrlamide gel electrophoresis).Another example includess " isoelectric focusing".In isoelectric focusing the protein is separated on the basis of its net charge.The main principle lies on the basis of finding isoelectric point i.e. at which the net charge on the protein is zero.The protein is loaded in the gel and then it separates itself on the basis of the charge.NEgatively charged on the negative side and positively gharged on the positive side and the neutral ones in the centre.
Can protein electrophoresis be done on horizontal submarine gel?
Yes it can. Check the scientific literature for details.
What are some applications for gel electrophoresis used?
Paternity testing and crime lab applications (DNA matching) etc
What has the author Neil S Harris written?
Neil S. Harris has written: 'Multiple myeloma and related serum protein disorders' -- subject(s): Electrophoresis, Myeloma Proteins, Analysis, Blood Proteins, Blood Protein Electrophoresis, Multiple Myeloma, Blood
The protein efficiency ratio assesses protein quality by?
comparing the weight gained in lab animals consuming a test protein with the weight in lab animals consuming a standardized (reference) protein
