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Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
yes it will ferment the mannitol and cause yellow color change.
Streptococcus cannot grow on MSA plates because MSA is a selective and differential media. It is selective because only certain microorganisms can grow on it due to its high NaCl content, in which this concentration - near 10% in MSA - has an inhibitory effect on most bacteria, such as Streptococci.
No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.
the bacteria dont ferment in MSA, there is no growth on the plat
MSA stands for mannitol salt agar. Streptococcus can not grow on this type of agar. This is because it has a very high salt content, which allows only certain microorganisms to grow in it.
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.
Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.
Yes
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
yes it will ferment the mannitol and cause yellow color change.
Streptococcus cannot grow on MSA plates because MSA is a selective and differential media. It is selective because only certain microorganisms can grow on it due to its high NaCl content, in which this concentration - near 10% in MSA - has an inhibitory effect on most bacteria, such as Streptococci.