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What properties of bacteria cells dictate if they will stain gram positive or gram negative?
Wiki User
∙ 13y ago
The Cell Wall.
Mainly the lipid content of the Cell Wall is responsible for that.
According to the content [quantity and composition] of lipids in the cell wall, we can divide the bacterial world into two:
1) More than 90% lipid on the surface of the cell wall (Gram -ve), and
2)less than 10% lipid on the exterior of the cell wall (Gram +ve).
Now lets talk about gram staining. You must be knowing the alcohol treatment step in gram staining. The alcohol dissolves the lipids.
so the effect of this on:
Gram -ve:- The 90 % of cell wall get dissolved.bcoz of which it looses the previously added stains.and it retain the only stain(pink colour) which is added after alcohol treatment.
Gram +ve:-only 10% cell wall get dissolve,so it looses only 10% of stain.So the bacteria still reatain more than 90% of stain (purple color) added before the alcohol treatment.
Wiki User
∙ 13y ago
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