Want this question answered?
No. restriction enzymes do not cut proteins. restriction enzymes cut DNA molecules at specific sites called restriction sites.
A restriction map plots restriction sites within a chain of DNA. You cannot create a restriction map without restriction enzymes. Restriction sites are points in a DNA molecule that contain certain strings of nucleotides, which can only be identified by restriction enzymes.
Children receive half of their genetic material from each parent. There are specific sites on DNA, known as restriction sites, that are recognized by restriction enzymes. These are used to determine paternity. Samples of DNA from the mother, father and child are taken. They are all digested ('cut') by the same restriction enzymes. These DNA fragments are then separated by gel electrophoresis (which separates fragments based on size). The bands of the child are compared to the mother and father's. If the band is not the same as the mother's, it must have come from the father. If these do not match up, then the sample was not taken from the biological father.
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
Restriction maps show the lengths of DNA fragments between restriction sites in a strand of DNA.
They are used to show the lengths of DNA fragments between restriction sites in a strand of DNA.
Let N = number of fragments: For circular DNA, the number of restriction sites= N For linear DNA, the number of restriction sites= N-1
RFLPs
restriction enzymes or endonuclease enzymes
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
No. restriction enzymes do not cut proteins. restriction enzymes cut DNA molecules at specific sites called restriction sites.
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
A restriction map plots restriction sites within a chain of DNA. You cannot create a restriction map without restriction enzymes. Restriction sites are points in a DNA molecule that contain certain strings of nucleotides, which can only be identified by restriction enzymes.
Children receive half of their genetic material from each parent. There are specific sites on DNA, known as restriction sites, that are recognized by restriction enzymes. These are used to determine paternity. Samples of DNA from the mother, father and child are taken. They are all digested ('cut') by the same restriction enzymes. These DNA fragments are then separated by gel electrophoresis (which separates fragments based on size). The bands of the child are compared to the mother and father's. If the band is not the same as the mother's, it must have come from the father. If these do not match up, then the sample was not taken from the biological father.
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
By the size of the DNA molecule. longer DNA molecules move slower, and shorter DNA molecules move faster!