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A restriction map plots restriction sites within a chain of DNA. You cannot create a restriction map without restriction enzymes. Restriction sites are points in a DNA molecule that contain certain strings of nucleotides, which can only be identified by restriction enzymes.
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What information is not given by a restriction map?
Restriction mapping is the most detailed thing that can be done with a segment of the DNA.It gives valuable detail about the gene regulating sequence and the introns.Restriction enzymes ans DNA ligase are important in making recombinant DNA.
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
Can Restriction Enzymes be used on all DNA?
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
What is restriction analysis?
restriction analysis is Comparison of DNA patterns.
How do restriction sites and a restriction map relate?
Restriction maps show the lengths of DNA fragments between restriction sites in a strand of DNA.
What do the bands on a restriction map show?
DNA sequence.
What information does a restriction map given about DNA?
it gives your mom
Which phrase is true for a DNA fingerprint?
it is a type of restriction map.
What information is not given by a restriction map?
Restriction mapping is the most detailed thing that can be done with a segment of the DNA.It gives valuable detail about the gene regulating sequence and the introns.Restriction enzymes ans DNA ligase are important in making recombinant DNA.
What part of speech is restriction?
Restriction is a noun.
How is DNA fingerprint a specific type of restriction map?
They both show the size of DNA fragment due to the number of repeats. They represent the uniqueness of a person's DNA.
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
Can Restriction Enzymes be used on all DNA?
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
Which pair of enzymes is necessary to make recombinant DNA?
Actually the answer would be Restriction enzyme and DNA ligase.
