I am doing a research right now with bacterial populations in the reproductive tract of ewes. I took some ordinary samples of bacteria from around the house and plated them on the sheep blood agar plates. I guess to answer your question...various types of bacteria can grow on these plates. The samples i took have patterns that could have been E. Coli, Streptococci and various other bacteria that had characteristics of hemolysis (alpha, beta and gamma). Are you looking for something specific?
Azide blood agar base contains sodium azide which has been proved to have a bacteriostatic effect on Gram-negative bacteria, thus this medium is used for the isolation of streptococci and staphylococci in clinical specimens, water, foods, etc. MacConkey agar is designed to grow Gram-negative bacteria and differentiate them for lactose fermentation. Nutrient agar is used for the routine cultivation of non-fastidious bacteria.
Many pathogens either fail to grow entirely or exhibit morphologies and hemolytic patterns on human blood agar that confound colony recognition. Availability of sheep blood is one reason (Need not to take consent to draw blood). Safety (free from HIV, HBV or HCV like organisms), another important reason. Blood from specially breed sheep for microbiological purpose are free from antibiotics which interrupt the growth of bacteria. Because of hemolysis which occur more frequently than to other animals blood, may be a reason but, not sure.
Because plate count agar is still agar. therefore, it still has all the nutrients that bacteria require to grow.
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
Agar and petri dishes.
Agar is a semi solid medium used to grow bacteria.
blood or nutrient agar would work, but blood agar will most likely grow more bacteria.
Bacteria will grow in blood but no the growing medium in petri dishes should be clear agar.
to inoculate an agar plate you would place bacteria on the agar to grow
It depends on the type of blood agar you are using. Blood agar generallly means whatever agar you are using + 5-10% of some type of mammalian blood (sheep, horse, rabbit, etc). I generally use TSA + 5% Sheep blood or Brucella Agar + 5% Horse blood, for instance.
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
Agar (a seaweed derivative)
Azide blood agar base contains sodium azide which has been proved to have a bacteriostatic effect on Gram-negative bacteria, thus this medium is used for the isolation of streptococci and staphylococci in clinical specimens, water, foods, etc. MacConkey agar is designed to grow Gram-negative bacteria and differentiate them for lactose fermentation. Nutrient agar is used for the routine cultivation of non-fastidious bacteria.
There are areas on the agar plate where no bacteria grow because that area was missed when the plate was streaked.
Many pathogens either fail to grow entirely or exhibit morphologies and hemolytic patterns on human blood agar that confound colony recognition. Availability of sheep blood is one reason (Need not to take consent to draw blood). Safety (free from HIV, HBV or HCV like organisms), another important reason. Blood from specially breed sheep for microbiological purpose are free from antibiotics which interrupt the growth of bacteria. Because of hemolysis which occur more frequently than to other animals blood, may be a reason but, not sure.
Agar is a solid form of nutrient for different strains of bacteria. Anyone who needs to grow bacteria for use in their labs or experiments would use agar.
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.