Streak plating and picking a single colony for the culturing in a broth.
The need of pure culture of bacteria to characterize an individual species. Pure culture are also important to study the morphology and physiology of individual bacterial species, their biochemical behaviour and response to different compounds like antibiotics, which all can me alter by the influence of other species if prestent (in mixed culture) and also for isolating and studying of their molicular structure i.e. DNA or RNA. Some common ways to obtain a pure culture of bacteria are: 1 The spread plate technique. 2 The pour plate. 3 Streak plate technique.
if contamination is noted in a subculture sample, what should be to obtain a pure culture
Its the only way to get a pure culture.
Robert koch
It gives microbiologists a way to grow bacteria on a solid medium. This is important if you want to be able to obtain a pure culture, without a solid medium to grow bacteria it would be nearly impossible. On a solid media a single colony can be isolated and used to innoculate a further plate or broth culture. All the bacteria present in a colony should be decendents of a single organism and so be genetically identical. To obtain a pure culture in liquid media, you would have to do numerous serial dilutions which is very labor intensive compared to streaking a solid medium. Even after many serial dilutions there would be no way to ensure a pure culture.
i obtain pure culture of bacteria from a mixed culture for obtain pure one bacterial culture
The need of pure culture of bacteria to characterize an individual species. Pure culture are also important to study the morphology and physiology of individual bacterial species, their biochemical behaviour and response to different compounds like antibiotics, which all can me alter by the influence of other species if prestent (in mixed culture) and also for isolating and studying of their molicular structure i.e. DNA or RNA. Some common ways to obtain a pure culture of bacteria are: 1 The spread plate technique. 2 The pour plate. 3 Streak plate technique.
if contamination is noted in a subculture sample, what should be to obtain a pure culture
Its the only way to get a pure culture.
control the contamination
Robert koch
simple distillation
Culture media should be specific to avoid contamination of unwanted microorganisms and to obtain a pure culture of microorganisms.
It gives microbiologists a way to grow bacteria on a solid medium. This is important if you want to be able to obtain a pure culture, without a solid medium to grow bacteria it would be nearly impossible. On a solid media a single colony can be isolated and used to innoculate a further plate or broth culture. All the bacteria present in a colony should be decendents of a single organism and so be genetically identical. To obtain a pure culture in liquid media, you would have to do numerous serial dilutions which is very labor intensive compared to streaking a solid medium. Even after many serial dilutions there would be no way to ensure a pure culture.
The main advantage of T- streak method: 1. To get a very good isolated colonies.2. To obtain pure culture from mixed culture.
The main advantage of T- streak method: 1. To get a very good isolated colonies.2. To obtain pure culture from mixed culture.
A pure culture is defined as a population containing only a single species or strain of bacteria. Contamination means that more than one species is present in a culture that is supposed to be pure.