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Q: What types of molecules will travel the longest distance in electrophoresis?
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If you have three molecules small medium and large which will travel through gel electrophoresis faster?

Small


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In regards to analyzing DNA with gel electrophoresis Are DNA fragments separated by size?

Yes, electrophoresis involves seperation depending upon size by applying charge to the DNA sample loaded which then travels form negative to positive eletrode as DNA being negatively charged. Thus the small sized molecules will travel faster as compared to larger molecules.


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Is DNA smaller or bigger than a protein?

Let's put it this way, we know that electrophoresis is a test for the sizes of the fragments of DNA molecules while SDS-page is a test of the size of protein molecules. If you use electrophoresis to test the differences of protein, there will not be any bands as all the protein will travel to the end of SDS-page. Therefore, we can conclude that the pores of electrophoresis is much more larger than SDS-page. Since electrophoresis has larger pores than SDS-page, it also shows that overall DNA is larger than protein in size.


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Which fragements travel the fastest and farthest during electrophoresis?

The smallest and lightest fragments.


In a gel electrophoresis's why are the lines closer together when the DNA strands are heavier?

Because the DNA cannot travel very long and spread apart when the strands are heavy. As a result, the DNA strands will all travel a relatively short distance and be closer together.


Function of agarose in agarose gel electrophoresis?

Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.


Does the car traveling the longest time always travel the greatest distance?

No. In general, for the simplified case of constant speed, use the formula: distance = speed x time