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Lack of desired sharpness in photos may be caused by the object being out of focus or movement of either the object or the camera. Using manual focus, a good lens and a tripod are a few ways to get sharper images.
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If an object appears blue on a thermal camera, the object does not have a significant heat source within it
The distance it will focus will depend upon the type of camera being used. It also depends upon which 300 mm lens you are using.
use the fine focus knob until we get sharp image
The fine focus knob is used to bring an object into fine focus, while the course focus knob is used to bring an object into approximate focus. The course focus knob should only be used on the low-power and scanning objectives of the light microscope. Using it on the high power microscope is okay, but you should be very careful. It is no recommended to use the course adjustment knob on the oil-emersion objective as it is so close to the stage and could lead to a potential break in the slide. Fine focus adjustment knob is useful to bring out the fine details in the specimen on your slide. Can be used on any of the objectives, but most receptive on the high-power and oil-emersion objectives.
What you do first when you use a microscope you put the slide in. Then you focus the microscope. Then you look at the object you are supposed to or look at the object you want to.
I can't answer to this specific camera, but I don't see why it wouldn't be the same as any other. You place the main subject within the target zone of the viewfinder (the zone is usually defined by framing lines or marks), depress the shutter button halfway to get the lens to lock in the focus, move the camera to compose as required, then press the shutter button the rest of the way to take the shot.
Coarse focus on a microscope is used only when you are using the lowest objective lens. You use it to get the object magnified mostly. The fine focus is then used to adjust the resolution to the viewer's eyesight.
Stabilizing the camera using a tripod will help to ensure that its able to stay in focus. As technology improves quickly though if the quality of the footage is low it might just be a good idea to invest in a new camera for recording.