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It was Francesco Redi's experiment. In 1664 Redi wrote his first work Osservazioni intorno alle vipere(Observations about the Viper). Redi is best known for, published in 1668 as Esperienze Intorno alla Generazione degl'Insetti (Experiments on the Generation of Insects).
It proved that flies did not spontaneous generate. He put meat in a flask and covered it with cheesecloth so that air could get in but not flies. The other flask was a control and did not have cheesecloth. Only the flask without cheesecloth had flies.
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