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Depending on what happened in the previous 2 games, the characters are varied slightly. When starting a new game, if you chose for Kaiden to live, then Ashley won't be available as a character and same goes for if you choose to let Ashley survive.

Available squadmates throughout the game are:

Garrus

Liara

Kaiden (or Ashley).

James

EDI - She will become available later in the game.

Tali'Zorah - She can only be a squadmate for a few missions and she will appear later on in the game.

Shepard's allies are:

Joker ('Jeff')

Engineer Adams

Admiral Hackett

Luitenant Cortez

Specialist Traynor

Dr. Karin Chakwas

Diana Allers - She is a reporter who will request interviews after certain missions. You can also choose for her to leave the Normandy.

Miranda.

The main enemies are the Illusive Man and Kai Leng.

See the related link for more information.

(You will have to scroll down to get to the Mass Effect 3 characters).

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