DNA Helicase.
Dna ligase -the enzyme which stitches them together into a single ,unfragmented daughter molecule is called dna ligas. Dna helicases-opening of the dna double helix ahead of the replication fork. Rna primase-synthesis of rna primers for dna chain elongation,the enzyme is a component of primosomes. Dna polymerase-dna replication is more accurately described as polymerization.the enzyme which catalyzes this polymerization is called,dna polymerase. Submit by anupriyachatterjee.
Topoisomerase
1. Helicases unwind the double helix at the replication fork and single strand binding proteins (SSBs) stablilize unwound DNA ahead of the fork.
The polymerase itself does not separate the DNA strands. Helicase (another enzyme, sometimes found in complex with a polymerase holoenzyme) does the separating for it, ahead of the replication fork.
DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via a polymerase chain reaction, also known as PCR. Creative Biogene
Dna ligase -the enzyme which stitches them together into a single ,unfragmented daughter molecule is called dna ligas. Dna helicases-opening of the dna double helix ahead of the replication fork. Rna primase-synthesis of rna primers for dna chain elongation,the enzyme is a component of primosomes. Dna polymerase-dna replication is more accurately described as polymerization.the enzyme which catalyzes this polymerization is called,dna polymerase. Submit by anupriyachatterjee.
Topoisomerase
The initiation complex makes a small gap for a helicase enzyme to bind. It is the helicase the 'undwinds' the DNA for most of replication. In E. coli, for example, DnaA protein binds DNA to make a small gap between the two DNA strands, where DnaB enzyme (a helicase) can bind to the lagging strand (the one that is copied in fragments). From there, DnaB unwinds the DNA ahead of the polymerase enzyme.
1. Helicases unwind the double helix at the replication fork and single strand binding proteins (SSBs) stablilize unwound DNA ahead of the fork.
The polymerase itself does not separate the DNA strands. Helicase (another enzyme, sometimes found in complex with a polymerase holoenzyme) does the separating for it, ahead of the replication fork.
It checks the DNA for errors
Catalyzes the transcription of DNADNA polymerases exist as dimers associated with the other necessary proteins at the replication fork and this representation is identified as the replisome. The template for the lagging strand is temporarily looped through the replisome such that the DNA polymerases are moving along both strands in the 3'---->5' direction simultaneously for short distances, up to the distance of an Okazaki fragment.As the replication forks progresses along the template strands, the newly synthesized daughter strands and parental template strands reform a DNA double helix. Hence it is explicit that that only a small stretch of the template duplex is single-stranded at any given time.The progression of the replication fork requires that the DNA ahead of the fork be continuously unwound. Since the eukaryotic chromosomal DNA is attached to a protein scaffold the progressive movement of the replication fork thus intruding severe torsional stress into the duplex ahead of the fork
The two strands of a DNA molecule are antiparallel to one another (the backbone of one strand runs from 5'-3' while the complimentary strand runs 3'-5'). Unfortunately, DNA polymerase, the enzyme responsible for replicating DNA, can only make DNA in a 5'-3' direction (and read DNA in the 3'-5' direction). Also, it needs a "primer" to give it a place to bind and start replication. So this creates a problem when synthesizing the 3'-5' stand because your enzyme will only synthesize 5'-3'. During replication this is solved by synthesizing small pieces of DNA ahead of the replication fork on the 5'-3' mother strand. Thus we have one daughter strand which is synthesized as a continuous piece of DNA (called the leading strand) and one daughter strand which is synthesized in small, discontinuous pieces (called the lagging strand). However, at the extreme end of the DNA, we run into another problem. The leading stand can be made to the very end, but the lagging strand cannot because you need the RNA primer upstream to begin each piece of the lagging strand DNA but at the end of the DNA there is nothing for this piece to attach to. Thus, the last section of the lagging strand cannot be synthesized and after several rounds of DNA replication, the DNA molecule gets smaller and smaller. This is "the end of replication problem" and it is solved by putting a DNA cap on the ends of DNA called a telomere which does not code for any protein, thus when this information is lost it does not have severe consequences for the cell.
DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via a polymerase chain reaction, also known as PCR. Creative Biogene
Topoisomerase: are isomerase enzymes that act on the topology of DNAHelicase untwists the double helix and separates the template DNA strands at the replication fork. This untwisting causes tighter twisting ahead of the replication fork, and topoisomerase helps relieve this strain
A five letter word for 'in front' is ahead.
"Lie ahead" is used for plural subjects, and "lies ahead" is used for singular subjects.Example:"Trouble lies ahead.""Troubles lie ahead."