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Optical spectrophotomerty
This technique prevents scratching of the cuvette in those areas through which the light will pass. Scratches on the cuvette can lead to erroneous measurements.
e-value of an optical brightening agent is the absorbance value obtained (in the UV region), when the absorbance of 1% solution is measured using a cuvette of 1 cm path length. T.J.R.PILLAI
A cuvette is, basically, a glorified test tube for use in a spectrometer. They're sometimes square in cross section to avoid refraction artifacts. Depending on what part of the spectrum is under consideration, they may be made of quartz, though plastic and glass cuvettes do exist. You put a liquid sample into it then put the cuvette into the instrument. The beams inside of the instrument can 'see through' the quartz and 'read' your sample.
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. the pathlength of light being the width of the cuvette and the extinctin coefficent being specific to the product molecule.
They are just tapers that react to a black light. Kind of like UV paint that glows.
1: control, recalibirate spectrophotometer 2: photosynthesis does not occur without light 3: photosynthesis does occur with light and live chloroplasts 4: boiling chloroplasts=no photosynthesis 5. control
Plastic and glass both absorb UV light. There are modern plastics that are now transparent to UV so plastic has begun to replace expensive quartz cells in UV spectrometry. These curvettes (cells) are transparent to UV down to 220 nm and have the advantage of being cheap enough to throw away after use. These are now used by biologists when analysing DNA/RNA or proteins. I don't know what chemists use but they tend to be traditionlists so probably still use quartz which has the advantage of being unreactive to most chemicals they can throw at them.
Cuvette
The absorption of sample at maximum wavelength will be reduced by1/2 . Vijay Bhoir (Flamingo pharmaceuticals Ltd.Rabale Navi Mumbai ).
it can cause skin cancer
electromagnetic radiation, from radio through UV.