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Which objective lens is the only lens that should be used with oil immersion?
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What would you observe if you forgot to use oil with the oil immersion lens?
Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
What does the high lens of a microscope only use?
That is the oil lens. For suspension of microbes in an oil solution in which the objective lens is also immersed. Improves resolution.
Why should you only use the fine adjustment when the high power objective is in position?
Because if you use the coarse adjustment under high power, there is a greater risk that you will crack the slide on the microscope and damage it. The fine adjustment only moves the objective lense small amounts and very slowly, so there is no chance that you will damage the slide.
Which objective should the coarse focus knob be used?
The coarse adjustment knob is only used with the low-power objective lens (usually 4x) because all the other ones are too long and the coarse adjustment knob may bring the slide up too qucikly, therefore it might break the slide or crush the specimen being observed. When using the medium (10x) and high (40x) power objective lens, it is best to use the fine adjustment knob to focus on the details instead.
What would you observe if you forgot to use oil with the oil immersion lens?
Immersion oil is only required when using a light manuscript at 1000 times magnification (10x from occular lens and 100x from objective lens). At 1000x magnification the image will have poor resolution (loook fuzzy) without the use of immersion oil. This is because the cover slip on the sample and air have different refractory indexes. The light scatter that occurs during the transition from glass to air is noticable at such a high magnification. Immersion oil has a refractive index very similar to the cover slip, thus reducing the light scatter as the light passes from the sample to the objective lens.
Why is it important to only use the fine adjustment knob when using the highest power objective lens?
When using high power, the lens is very close to the sample - and so you risk damaging the slide and/or lens if they touch - therefore you only want to move the platform a little bit at a time. It is very similar with an oil immersion - you don't want to damage the lens by allowing the sample to touch it.
When would you need to use the oil immersion lens?
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
What is the maximum magnification of the dark-field microscope?
Most go up to 1000x as it is light field microscopy. The ocular lens (the one that you look into) is 10x, but there are different objectives to focus on the specimen that you rotate to chose. The lowest is usually 4x, then 10x, 40x, and then 100x. Multiply ocular lens (10x) X objective lens you are using (ex: 40x) = Total 400x Oil immersion drop is used ONLY on the 100x objective.
Why should YOU Only use THE fine adjust when the high power objective IS in positions?
So you don't crack the slide or damage the lens
What does the high lens of a microscope only use?
That is the oil lens. For suspension of microbes in an oil solution in which the objective lens is also immersed. Improves resolution.
How can you tell which objective lens is in the viewing position?
When the objective lens you want to use is in the viewing area it will click in place. At times, trial and error will help. It will only take minute or two to get it correct.
Why should you use the fine focus knob only when working with the 40x objective lens?
You should have already used the Coarse adjustment on 10x so when you switch to 40x, all you need to do is fine adjust it. Plus, you don't want to break it by turning the Coarse up all the way.
What precautions must be taken when using a microscope when it is in high power lens?
Because you are at the highest magnification, only turn the fine adjustment knob when you are using the high-powered objective lens, and not the coarse adjustment. Otherwise you risk getting your sample out of focus, and you will probably only be able to refocusby starting with the lowest power objective lens.
Why should you only use the fine adjustment when the high power objective is in position?
Because if you use the coarse adjustment under high power, there is a greater risk that you will crack the slide on the microscope and damage it. The fine adjustment only moves the objective lense small amounts and very slowly, so there is no chance that you will damage the slide.
How was the compound microscope different from leeuwenhoeks?
Compound microscopes have more than one lens. The first (objective) lens magnifies the object to produce an image. Subsequent lenses magnify an image produced by a previous lens. Leeuwenhoek's "microscope" had only one lens -- simple magnification only. ~The Undertaker~
Why should lens paper only be used once?
lens paper is fragile
