what is serial dilution and spread plate technique
While lysozyme will break down the cell membrane of some gram positive bacteira, it does not affect all gram positives therefore it is not a suitable means of separating the two. Serial dilution would be a much more effective method.
The total count includes dead cells as well as living cells.
Because the osmotic pressure of "plain" water can be too much for bacteria, causing them to pop, and throw off your colony counts during your serial dilutions. A buffered saline solution keeps the bacteria at their usual osmotic pressure. Typical saline is 0.85%.
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Serial Dilution Plate Technique is the method to estimate soil microbial population by Dhinga & Sinclair in 1993.
what is serial dilution and spread plate technique
Serial dilution technique
A common design for estimating the concentrations of compounds in biological samples is the serial dilution assay, in which measurements are taken at several different dilutions of a sample, giving several opportunities for an accurate measurement. Curren tly, serial dilution is a standard tool in the fields of toxicology and immunology.Serial dilution helps to choose a dilution which is relevant to our experiment.Often the standard which is given to you in the lab is far to strong for the experiment and it needs to be diluted. But equally the equipment has a detection limit so we can't dilute it to much, or if it is too diluted the experiment might not work.
Both force you to read your lab manual.
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
Eisner Hewer invented traditional method in math
1:5
the total count includes dead as well as living cells
dilute it 1 in 5000. likely best done with a serial or step dilution
Henry Ford invented the assembly-line method of production
While lysozyme will break down the cell membrane of some gram positive bacteira, it does not affect all gram positives therefore it is not a suitable means of separating the two. Serial dilution would be a much more effective method.