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Q: Why agar must use in spread plate?
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How can you verify the purity of bacteria?

The only way i can think of is using a spread plate technique to observe what grows on an agar plate. Capillary electrophoresis I believe is use to split biological samples (and chemical) based on charge and size.


Why do you use saline water when preparing a plate with blood agar?

TONICITY


What is the significance of using a 'dry and hard' agar plate in streak plating?

Why is it impotant to use dry and hard agar for streaking


Why must use starch casein nitrate agar and what is the starch casein agar properties?

Because it is a selective medium


How do you recognize bacteria on agar plate and not confusing bacteria with eukaryotes like yeast or fungi?

Use selective media agar plates. Different types of agar will let bacteria grow and inhibit fungal growth, or vice versa.


Why must we use sterile techniques to incubate agar plates?

Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.


If you had to observe the detailed morphology of specise of mold hyphae is it better to use agar plate or henrici slide?

i believe henrici slide


What specific medium must be use in testing the effectiveness of antibiotics?

A growth medium must be used. The most common is Mueller-Hinton agar, but potato dextrose agar or other growth media could also be used.


What is the difference between himedia ready to use nutrient agar plate and nutrient agar plates prepared by dehydrated media?

Ready to use plates are more expensive because the main difference between the two is the amount of work you have to do. Already made plates and ready to go. Dehydrated you need to make the agar from scratch and pour into empty petri, and wait for it to solidify before you can use them.


How can organisms that dont use starch grow on a starch agar plate?

Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.


What is the use of agar?

agar slant - save space (advantage) - suitable for long-term storage agar plate - short-term storage - used for assays Broth culture - ... agar slant - save space (advantage) - suitable for long-term storage agar plate - short-term storage - used for assays Broth culture - ...


How does campylobacter jejuni appear when cultured on campylobacter agar base?

It depends on which Campylobacter agar base addatives you use - there are many variations. Typically the colonies round or have slightly irregular edges, they are white when small and spread to become transparent later.