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Fermentable Carbohydrate Depletion...

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13y ago
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6mo ago

TSI (triple sugar iron) test observations are made 18 to 24 hours after incubation because this time frame allows for the characteristic reactions to occur. The test is designed to differentiate between bacteria that can ferment glucose, lactose, and/or sucrose and produce acid and gas. Observing the reactions within this time frame ensures accurate interpretation of the test results.

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Q: Why are TSI test observations made 18 to 24 hours after incubation?
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What term describes an experiment done in a test tube?

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Doing so will ensure that the carbohydrates substrates have not benn depleted and that deradation of peptones yielding alkaline end products has not taken place.


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