Why are cultures collected from the human body usually incubated at 35 to 37c?

Answer 1

Hi

what you incubate is generally E.coli at 37C. The optimum growth temperature for this bacteria is 37, although if you are expressing some specific recombinant protein the induction temperature may very depending upon the promoter of recombinant gene, but the general temp for bacterial growth is 37C.

There are other bacteria like Listeria Monocytogenes which grow at 8C, also, after appropriate growth of E.coli sometimes you change temperature or can do other things depending upon you promoter for recombinant gene in it.

So bottom line generally 37C for growth, but also depends on bacteria in very few cases for purpose of biotechnology.

hope this will help you.

Answer 2

At 37 degress the bodies enzymes work at their optimum temperature and the most reactions take place. There are more frequent collisions of enzymes and their substrates to the active site of the enzymes. The enzymes digest all of the food and catalyse most of the reactions in the body

Answer 3

I guess because of health/hazards around growing bacteria. The heat also plays a factor. Also your teacher may not have permission to do this activity, teachers have a schedule in what they teach.

Answer 4

that is usually the temperature of a normal human's body

Answer 5

at 37 degrees the enzymes are denatured