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Q: Why are motile bacteria able to move outwards in motility test agar away from the needle inoculation line?
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How is SIM medium used to detect motility?

SIM agar may be used to detect motile organisms. Motility is recognized when culture growth (turbidity) of flagellated organisms is not restricted to the line of inoculation. Growth of non-motile organisms is confined to the line of inoculation.


Are bacteria growing in the semi solid agar deep motile?

Yes. If the bacteria has spread from the inoculation point, the bacteria is motile. It will look like a upside down Christmas tree.


Can non motile bacteria move?

Yes, the microbes will exhibit either brownian movement or true motility.


What aspect of microbial properties can be observed via stab inoculation technique?

The motility of a microorganism can be observed. Motile organisms, using the flagellum, will move away from the stab line, hence will appear to have "diffused" into the medium. Non-motile organisms will remain in the stab line.


Does Ecoli have a true motility?

Motile according to the Motility-Indole-Ornithine Test.


Which of the following bacteria exhibit true motility on the slides among B.cereus P.aeruginosa and S.volutans?

They all have flagella and therefore are considered to be motile.


If sim medium was used for motility determination for Proteus vulgaris what noticeable change to the medium will be observed?

It will turn black in the inoculation stab as well as throughout the medium because P. vulgaris is a flagellated and motile organism.


Is a non motile organism over inoculation of the slide with organisms a false negative?

If a slide is over-inoculated, it means that there are too many samples on one slide. This means that it can give a false negative regarding the motility of the organism in question.


Is Bacillus subtilis motile or not?

Yes it is. The reason Bacillus is motile is because it is an aerobe. This means it thrives on oxygen. Inside the agar, oxygen is limited and usually it will move toward the surface where it is more oxygenated. Motility of Bacillus depends on the incubation time, environment and preparation of the agar. If all the other tests point towards Bacillus subtilis, you should be just fine.


Are animilia motile or nonmotile?

Mastigophores use flagella for motility.


What the other method used to determine motility?

You can do an experiment to determine motility. You use a medium of agar that has tetrazolium salt (TTC). The TTC serves as a terminal electron acceptor for the bacteria and turns the medium red. You would inoculate a needle with your bacteria and put it straight into the media and straight out. After incubating your bacteria, if you see the red move away from where you inserted the needle, then it would indicate that your bacteria is motile. If the red is only in the place where you inserted the needle, then it is not motile.


Where do the spermatozoa mature and become motile?

The locomotion of the flagellum produces motility.