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Q: Why auxin and cytokinin ratio is more important for callus culture?
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What media is recommended for tissue culture of alstonia scholaris?

For the tissue culture/callus culture of Alstonia scholaris MS media supplemented with a balanced concentrations of 2,4-D(auxin) and FAP(cytokinin) can be used.The concentrations of auxin/cytokinin are*****mg/l [(kumarsumit90@gmail.com :) ;)]


Direct and indirect morphogenesis in Plant tissue culture?

Callogenesis is the shoot development in callus, due to low auxin and hign cytokinin concentration in the culturing medium during tissue culture.


What are the five plants?

Auxin,Cytokinin,Abscic Acids,Gibberellin,&Ethylene


How do you isolate a plant cell?

A plant cell can be isolated by using different priscribed techniques keeping in mind the sourse of isolation. For eg. to isolate a leaf cell first:- collect the leaf, homogenase it (crush it) in proper medium then add the mixture in centrifugation tube and centrifuge it repeatedly for 2 to 3 times. discard the supernetant and inoculate the debris in suitable culture media keeping the auxin and cytokinin ratio equal. CALLUS formation take place which is mass of undifferentiated cells. Now from this callus we can isolate plant cells.


What is thidiazuron?

It is plant growth regulator. it is a cytokinin. Cytokinin is involved in both local and long distance signalling; as a long distance signal CK shares the same transport systems used by the plant for moving purines and nucleosides. Cytokinins are involved in many plant processes, including cell division, shoot and root morphogenesis, chloroplast maturation, cell enlargement, auxiliary bud release and senescence. The ratio of auxin to cytokinin is crucial during cell division and the differentiation of plant tissues and auxin is known to regulate the biosynthesis of cytokinin


Are plant hormones an example of an internal stimuli?

Yes many plant hormones are secreted by plants internally. Such as auxin, gibbrailin, cytokinin etc.


What are the 4 hormones used in horticulture?

Auxin,Cytokinin,Abscic Acids,Gibberellin,&Ethylene


What effects does auxin have on plants?

auxin plays an important role in the growth of meristemetic tissue so as to increase the height of plant.


What hormone causes plants to grow toward light?

Auxin is a chemical which makes plants grow faster.Specifically, the chemical in question can be described as a plant hormone or phytohormone to regulate growth. The terms comes from the Greek word αυξειν (auxein) for "to grow, to increase." Auxin may occur naturally -- often in cooperation with the plant hormone cytokinin -- or synthetically.


Crop physiological role of cytokinin?

Cytokinin are made in greatest amounts in the roots. Simply put, they may be an indication that things are going well for the root and shoot growth may therefore commence. The principle roles of the root are to take in water and minerals from the soil, to anchor the plant and to store nutrients like starch that has been made by the leaves (the last in some but not all plants). Since it's possible that salicylic acid acting as a hormone may be an indicator of an abundance of water, cytokinin may indicate that the plant is taking in more than enough minerals from the soil to just survive, so that it is OK to start growing more stems and leaves. In fact cytokinin inhibits senescence of leaves, and promotes new stem growth. Whether it needs a high level of salicylic acid also as indicator to give the green light to stem growth, is a good question... It's also quit probable that even if the plant is taking in good amounts of water and minerals from the roots they would not make salicylic acid and cytokinin if the root were under duress for other reasons, say by infestation, flooding or dislodging. In other words any condition that plant is in where it might be good to wait to start growth until a better situation is achieved. It's possible cytokinin is still made under these circumstances but the minerals are instead store for a better day. One last possibility is cytokinin alone indicates root prospering just as perhaps auxin alone indicates stem prospering. The reason for saying this is that cloning a single isolated plant cell into a full new plant, seems to require only cytokinin and auxin, not the additional salicylic and jasmonic acid (jasmonate is proposed in this present author's scheme as a sugar indicator with Auxin relegated to a role as a carbon dioxide and oxygen uptake indicator). Mind you most scientist woulds consider this entire answer highly speculative.


What is the relationship between auxin and phototropism?

Phototropism is the biological process for auxin's.


What is the chemical produced growing in root tips?

Auxin hormone