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What is the colour of bromophenol blue when neutral?
Bromophenol blue is green when neutral.
If you add a bromophenol blue solution to .01M HCl what solution color would result after mixing?
The bromophenol blue will turn yellow in the presence of an acidic solution like 0.01M HCl.
What color is bromophenol blue?
Bromophenol blue is a pH indicator dye that changes color depending on the acidity of the solution. In an acidic environment, it appears yellow, while in a neutral to alkaline environment, it appears blue.
Can you use bromophenol blue in place of bromothymol blue?
No, bromophenol blue and bromothymol blue are not interchangeable. They have different pH ranges for color changes and may not give accurate results if substituted for each other in experiments or procedures.
What happens if you mix bromphenol blue and NaOH?
When bromophenol blue is mixed with NaOH, the solution turns blue because the pH becomes alkaline. Bromophenol blue is a pH indicator that changes color in different pH ranges. In the presence of NaOH, which is a base, the bromophenol blue changes from yellow (at acidic pH) to blue (at alkaline pH).
What is the colour of bromophenol blue when neutral?
Bromophenol blue is green when neutral.
If you add a bromophenol blue solution to .01M HCl what solution color would result after mixing?
The bromophenol blue will turn yellow in the presence of an acidic solution like 0.01M HCl.
Can you use bromophenol blue in place of bromothymol blue?
No, bromophenol blue and bromothymol blue are not interchangeable. They have different pH ranges for color changes and may not give accurate results if substituted for each other in experiments or procedures.
What color is bromophenol blue?
Bromophenol blue is a pH indicator dye that changes color depending on the acidity of the solution. In an acidic environment, it appears yellow, while in a neutral to alkaline environment, it appears blue.
What happens if you mix bromphenol blue and NaOH?
When bromophenol blue is mixed with NaOH, the solution turns blue because the pH becomes alkaline. Bromophenol blue is a pH indicator that changes color in different pH ranges. In the presence of NaOH, which is a base, the bromophenol blue changes from yellow (at acidic pH) to blue (at alkaline pH).
Why you put bromophenol blue in lysis buffer?
Bromophenol blue is added to lysis buffer as a tracking dye to monitor the progress of protein electrophoresis. It helps visualize the sample migration through the gel during SDS-PAGE by imparting a blue color to the proteins.
Does Amax of bromophenol blue vary with concentration?
Yes, the absorbance maximum (Amax) of bromophenol blue does vary with concentration. As the concentration of bromophenol blue increases, the Amax shifts from its initial value. This change in Amax can be used to determine the concentration of bromophenol blue in a solution through spectrophotometric analysis.
Which is more polar bromophenol blue or phenolphthalein?
Phenolphthalein is more polar than bromophenol blue due to its structure and functional groups. Phenolphthalein contains more oxygen atoms that can participate in hydrogen bonding, making it a more polar compound compared to bromophenol blue.
What does dye contain?
The loading dye comprises bromophenol blue, Ficoll 400 and water majorly while Xylene cyanol, Tris and EDTA are optional in it. Bromophenol blue is one of the most popular indicators of DNA in agarose gel electrophoresis. Bromophenol blue is a pH indicator.
What is the pka of bromophenol blue?
Phenols are acidic substances (pH over 7).
Can you use bromothymol blue in place of bromophenol blue?
no.heres why:bromophenol-blue-1bromothymol-blue-1
What can you use in place of bromophenol blue?
Methyl orange or phenolphthalein can be used as alternatives to bromophenol blue in certain experiments requiring a pH indicator. It is important to consider the specific requirements of the experiment and the pH range of the indicator being used.
