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Why can't Enzyme panel reactions be used as the only source of rule-outs during antibody identification?
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In indirect elisa the enzyme liked antibody will attach to?
Another antibody that is attached to the epitope of interest.
Materials that slows down chemical reactions?
In biological chemical reactions, examples of these are called enzyme inhibitors. Enzymes speed up reactions, but enzyme inhibitors slow them down. This can be by either competing with the reactants for a spot on the enzyme, or by altering the enzyme's structure so that it does not speed up reactions anymore. In either case, enzyme inhibitors slow down chemical reactions.
How an ELISA works?
ELISA means enzyme linked immunosorbent assay. Let us keep it simple and describe a direct ELISA. First; a well plate is coated on the bottom of the well with an antigen epitope of interest. Then an antibody is prepared with an enzyme linked to it. Then the antibody is put into the well with a amount of neutral solution. The well is washed. Then the substrate of the antibody is put into the solution. If the antibody attached to the epitope was not washed away the enzyme will react with its substrate and this reaction will color the solution.
What does the shape of an enzyme have to do with how well the enzyme works?
The shape allows the enzyme to carry out specific chemical reactions.
What are the names of proteins that speed up chemical reactions?
Enzyme.
In indirect elisa the enzyme liked antibody will attach to?
Another antibody that is attached to the epitope of interest.
Enzyme antibody hormone and muscle are roles played by?
proteins
Materials that slows down chemical reactions?
In biological chemical reactions, examples of these are called enzyme inhibitors. Enzymes speed up reactions, but enzyme inhibitors slow them down. This can be by either competing with the reactants for a spot on the enzyme, or by altering the enzyme's structure so that it does not speed up reactions anymore. In either case, enzyme inhibitors slow down chemical reactions.
What ia an enzyme?
an enzyme is protien that catalyzes chemical reactions for organisms
What is a protein that changes the rate of chemical reactions?
its called an enzyme
How an ELISA works?
ELISA means enzyme linked immunosorbent assay. Let us keep it simple and describe a direct ELISA. First; a well plate is coated on the bottom of the well with an antigen epitope of interest. Then an antibody is prepared with an enzyme linked to it. Then the antibody is put into the well with a amount of neutral solution. The well is washed. Then the substrate of the antibody is put into the solution. If the antibody attached to the epitope was not washed away the enzyme will react with its substrate and this reaction will color the solution.
What does the shape of an enzyme have to do with how well the enzyme works?
The shape allows the enzyme to carry out specific chemical reactions.
What are the names of proteins that speed up chemical reactions?
Enzyme.
Is an enzyme a source of energy for endergonic reactions?
an enzyme is a protein that catalyzes (increases the rates of) reactions by lowering the activation energy required for the reaction. enzymes catalyze all kinds of reactions, including endergonic reactions.
What kind of chemical reactions speed up enzyme catalysis?
This varies per enzyme and what it catalyzes but chemical reactions that result in an increase in temperature often speed up enzyme catalysis.
What are the Various enzyme used in ELISA?
There is just one enzyme used in the ELISA reaction. This enzyme is linked to the secondary antibody. Commonly used ELISA enzymes are:Alkaline phosphataseHorseradish peroxidase
Why is the secondary antibody used in an ELISA test conjugated with an enzyme?
So that when the substrate is added, the reaction between the enzyme and the substrate will cause a change in color
