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Trichloroacetic acid is used for precipitation of the DNA during its extraction.
yes
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
The 200-400 mesh size is best for DNA extraction. The smaller sizes are usually used for metal ion extraction.
55-60oC
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
yes
turtle
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
ammonium acetae use to percipitate DNA from water.
The 200-400 mesh size is best for DNA extraction. The smaller sizes are usually used for metal ion extraction.
55-60oC
Perhaps the most basic precaution is to make sure that the warm water bath that will be used has a temperature that doesn't exceed 90C so as to denature only the protein component of crude extract and not the DNA which is the target isolate.
This question gave me cancer.
Triton X-100 is used as a lysis buffer for DNA separation.
It serves to break the tissue apart so the DNA can be subsequently extracted.
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.