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Because the retention coefficients of different substances are also different.
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What is chromatography fluid?
It refers to a set of technique used to separate different compounds. So involves separating chemicals and identifying them by color. Various chromatography products are used during the process.
What is mobile and stationary phase in Chromatography?
Moblie phase is that phase in chromatography that moves the analyte components along with it. Stationary phase remains static during chromatography.
Who discovered candy chromatography?
Chromatography was discovered by Ms.Felicetti & Sherlynne & Emily In 2011 during the investigation of a Science Fair Project
Do carotene's help in the capture of sunlight during photosynthesis?
Yes they do, carotenes have a different wavelength peak of light absorption than chlorophyll pigments. They are classified as secondary pigments.
What are the pigments formed during the Maillard reaction are called?
You think probable to components of caramel.
3 Why do the pigments become separated during the development of the chromatogram?
The chromatogram, or chromatography paper has solvent on it. Each pigment will separate itself from the others due to the relative solubility of that particular pigment.
Why are the plates in column chromatography called 'theoretical plates'?
The number of theoretical plates in a chromatography column is a measure of how "long" the column is - how well it separates. A "short" column will only separate large or heavy molecules, and the medium and light stuff is still mixed together in the last band. A "long" column will separate the little stuff better because there are more theorectical plates. Picture a stack of sieves with smaller and smaller holes as the column gets "longer" and you've got the idea. This "length" has virtually nothing to do with the physical length of the separating column. It is a function of the packing materials and solvents used during a separation.
Why is it important to avoid air bubbles in the column during chromatography?
It is important to avoid air bubles in the column during chromatography because you get a clearer picture and will see what ishappening alot clearer without problems.
What is the function of elution buffer in the DNA extration?
In column chromatography, it is put in the column to basically cleanse and lubricate. Generally, it helps to wash out any left-over proteins from a previous experiment. It can also help to separate the fractions that are collected.
What is chromatography fluid?
It refers to a set of technique used to separate different compounds. So involves separating chemicals and identifying them by color. Various chromatography products are used during the process.
Difference Rf values for monosaccarides and disaccharides?
Monosaccarides contain fewer polar covalent glycosidic bonds than polysaccharides and therefore move further up during thin layer chromatography and column chromatography.
What is mobile and stationary phase in Chromatography?
Moblie phase is that phase in chromatography that moves the analyte components along with it. Stationary phase remains static during chromatography.
Who discovered candy chromatography?
Chromatography was discovered by Ms.Felicetti & Sherlynne & Emily In 2011 during the investigation of a Science Fair Project
What do pigments absorb during photosynthesis?
light energy
Describe why you see fall colors during the fall season What happens to the green pigments that allows for the orange red and yellow pigments to be more visible?
Because the amount of sunlight is less and the weather is colder, trees allows the beta carotene (orange pigments), and other pigments, to be more visible compared to when the leaves have more chlorophyll (green pigments).
What substance that absorbs light?
Chlorophyll pigments absorb light during photosynthesis.
What is mean by isothermal in gas chromatography?
It means that you keep the temperature constant during the entire run
