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If the PCR that was run was an RT-PCR then the band with 300 extra bp could be caused by the presence of contaminating gDNA in the reaction. Many primers for RT-PCR are designed to sit in different exons. If the intron in between was about 300bp in length and gDNA was added to the reaction as well as cDNA then two bands would result, the shorter/lighter one from the cDNA and the longer/heavier band from the gDNA.
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What pairs differ by only a proton?
Conjugated pair of acid / base
What nitrogen base pair bondings are possible?
In DNA, adenine pairs with thymine, and guanine pairs with cytosine. In RNA, adenine pairs with uracil, and guanine pairs with cytosine.
Are AT base pairs or GC base pairs more stable?
A-T base pairs have 2 hydrogen bonds and G-C base pairs have 3 hydrogen bonds. Therefore, A-T base pairs are weaker than G-C base pairs.
What base in RNA pairs with adenine-?
Uracil is the base in RNA that pairs with adenine.
When purine base only pairs with a pyrimidine?
when a purine base only pairs with a pyrimidine
Give the products what acid base reactions and identify the conjugate acid base pairs NH3 H2O and NH4 CN?
NH3 + H20 <----> NH4+ + OH- Ammonia is a weak base so it is the favored side of the equilbrium. Conjugate acid and base pairs only differ by a proton. So ammonia and ammonium are pairs and water and hydroxide ions are pairs. NH4+ + CN- <-------> HCN + NH3
How many base pairs differ from person to person?
Alot of person's
What pairs differ by only a proton?
Conjugated pair of acid / base
What nitrogen base pair bondings are possible?
In DNA, adenine pairs with thymine, and guanine pairs with cytosine. In RNA, adenine pairs with uracil, and guanine pairs with cytosine.
How is acids and base differ from salts?
Salts are the products of reactions between acids and bases.
Are AT base pairs or GC base pairs more stable?
A-T base pairs have 2 hydrogen bonds and G-C base pairs have 3 hydrogen bonds. Therefore, A-T base pairs are weaker than G-C base pairs.
What are base pairs in biotechnology?
DNA base pairs.
How long are the base pairs of Okazaki fragments?
50 base pairs
What base in RNA pairs with adenine-?
Uracil is the base in RNA that pairs with adenine.
How many base pairs are in human sperm DNA?
four base pairs
How many base pairs are in Klinefelter's syndrome?
there are five base pairs in klinefelter syndrome
When purine base only pairs with a pyrimidine?
when a purine base only pairs with a pyrimidine
