0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
Is there any calculation to make phosphate buffer at pH 7?
Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html
What is the need for an ammonical buffer solution in the determination of hardness of water by edta titration?
during the complexometric titration using edta it is very necessary to maintain the ph of the solution near about 10 so we use ammonium chloride buffer if we will not use this buffer dring the titration ph of sol. will ho lower side
Can i use saline citrate buffer for DNA extraction from tissue of bivalve?
yes??
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Why you use pH 10 buffer in EDTA titrtion?
because EBT work best in that pH
If use sodium phosphate buffer instead of potassium phosphate buffer?
its ok to use
What does DNA polymerases use as their substrate?
The main substrate for for Taq polymerase is magnesium chloride
How do you prepare pH buffer soultion?
Decide on the concentration of the buffer, use 1L to be simple PH for your buffer should be within one pH unit from the pKa of the acid/conjugate base use Henderson Hasselbalch Equation pH = pKa + log ([Base]/[Acid]) For a 1 M buffer [Acid] + [Base] = 1
What is the function of TNE buffer?
We use it for isolation of proteins from yeast cells as a lysis buffer
Which bjt transistor amplifier can be used as a voltage buffer?
the common collector can use as voltage buffer
What is the advantages of buffer circuits in digital electronics?
buffer circuits helpful in overcoming the impedance matching problem.for example if we want to send a signal from one devise to other ,if there is no impedance matching between this two then signal is not transfered.now if we use buffer in between these two devises then the buffer without changing the signal shape or value it simply transffers the signal
What is the use of buffer?
To maintain constant the pH of a solution.
Why gets function gives a warning every time you compile a c program?
It is unsafe. In order to use gets() safely, you need to know how many characters you will be reading to ensure your character buffer is large enough: char buffer[10]; while (gets (buffer) != 0) { ...process buffer... } The above code has undefined behaviour when the number of characters read is 10 or more (you need one character for the null-terminator). This is because the character buffer, str, decays to a pointer (referencing &str[0]) and the function, gets(), cannot determine the number of characters in a buffer by its pointer alone. The gets() function was dropped from the C standard in 2011, however some implementations still include it. To avoid the warning, use the fgets() function instead. This allows you to specify the length of your buffer and (when used correctly) prevents buffer overflow. char buffer[10]; while (fgets (buffer, 10, stdin) != 0) { ...process buffer... }
What is the enzyme that synthesized DNA used in PCR that distinguishes it from the equivalent enzyme that carry out the same function in our cells or those of most bacteria?
PCR uses Taq polymerase (a type of DNA polymerase that comes from the thermophilic bacterium, Thermus aquaticus).The difference between that version of the enzyme and the one that our cells use is that Taq polymerase functions at much higher temperatures, allowing it to withstand PCR temperatures for thermal cycling.
How do you use buffer in a sentence?
You didn't say which meaning you want to use for this word. A buffer can be a barrier which lessens or absorbs a shock. The airbag acts as a buffer in case of an accident. She was the buffer between them, and kept the argument from getting out of hand. Buffer can also mean a chemical substance which minimizes the acidity of a substance. I need a bottle of buffered aspirin; the buffer keeps it from upsetting your stomach. Buffer can also be a verb meaning to do either of the above. The neutral zone between the two countries buffered their rivalry. That substance buffers the chemicals.
How do you increase buffer capacity?
Increase the concentration of salt and acid or base. If you are not suppose to increase concentration use more volume of buffer.
Is there any calculation to make phosphate buffer at pH 7?
Yes you need to know what molarity of the phosphate buffer you need to make and what voluime, then you can use Henderson-Hasselbalch equation. or simply use the phophate buffer calculator http://home.fuse.net/clymer/buffers/phos2.html
