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Could you use human polymerase for the extension step in the PCR reaction?
Wiki User
∙ 7y ago
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Wiki User
∙ 7y ago
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Can you eat fireworks?
They look interesting and are an extension of human creativity.
How is simulated blood typing activity similar to actual human blood typing?
In both simulated and actual human blood typing, there is an antigen-antibody reaction. A simulated agglutination reaction shows up the antigen.
What is normal human reaction time?
the measure of how fast products are made in a reaction
What is the reaction of the human body to gamma rays?
Gamma rays can cause radiation sickness and cancer in humans.
Is burning of gasoline in the car a chemical change?
Gasoline burning is an oxidation reaction, a reaction with oxygen.So, burning is a chemical change. And fire was the most important discovery of human beings.
Why taq polymerase is thermostable?
Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
Which best describes the purpose of the polymerase chain reaction (PCR)?
Polymerase Chain Reaction is widely used in many areas to identify DNA and detect infectious organisms or genetic variations, including the viruses that cause AIDS, hepatitis, and tuberculosis, detection of mutations in human genes, and numerous other tasks.
Where is polymerase in the human body?
kaka
What is defined as rt pcr?
This stands for reverse transcription polymerase chain reaction and pretty much means cloning. It's the process of recreating strands of human or animal DNA.
Which best describes the purpose of the polymerase chain reaction (PCR)?
Polymerase Chain Reaction is widely used in many areas to identify DNA and detect infectious organisms or genetic variations, including the viruses that cause AIDS, hepatitis, and tuberculosis, detection of mutations in human genes, and numerous other tasks.
What does the cytoplasmic extension in a human cell consist of?
The cytoplasmic extension in a human cell consists of collagen.
A small amount of DNA obtained from a mummy or frozen remains of a human may be cloned in order to clone small amounts of DNA what needs to be used to generate larger qualities of DNA?
To ramp up the DNA into a large enough amount to work with you would use polymerase chain reaction technique. PCR. By using the enzymes found in certain extremeophiles one alternately heats and cools the DNA solution with this extremeophile polymerase included and it ramps up the amount of DNA so you have a useful bit of DNA for insertion into a cloning vector.
Comprehension and extension example?
"girl"= comprehension (meaning): female human extension (to where it refers): little woman
What has the author Ede Wilson written?
Ede Wilson has written: 'Studies of human RNA polymerase II holoenzyme'
What is the action of the epitrochlearis?
In human beings, this helps in the extension of the forearm. If you are doing a cat dissection, then it helps in the extension of the forelimb.
What are some factors that affect human reaction rates?
Factors that affect human reaction rates include age, physical condition, level of fatigue, state of mind, distractions, and presence of drugs or alcohol. Additionally, the complexity of the task being performed can also impact reaction times.
What is the need to discover aliens?
An extension of natural human curiosity.
