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Taq Polymerase is an important enzyme component involved in the PCR reaction. Its A DNA polymerase and its role is to elongate the growing strands of DNA during the extension process. Since the Extension process in a PCR works at a temperature which a human DNA polymerase cannot remain active, the Taq polymerase obtained from Thermus aquaticus (living in the hot springs) are used and hence these enzymes are thermo stable.
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Why taq polymerase is strored at very low temperature as it is thermostable?
to store taq DNA polymerase at low temperature is to make it viable or we can say inactive for sometime. it doesnt die or decay but remains viable.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
What does DNA polymerases use as their substrate?
The main substrate for for Taq polymerase is magnesium chloride
What is the enzyme that synthesized DNA used in PCR that distinguishes it from the equivalent enzyme that carry out the same function in our cells or those of most bacteria?
PCR uses Taq polymerase (a type of DNA polymerase that comes from the thermophilic bacterium, Thermus aquaticus).The difference between that version of the enzyme and the one that our cells use is that Taq polymerase functions at much higher temperatures, allowing it to withstand PCR temperatures for thermal cycling.
How did Taq DNA polymerase acquire its name?
The Taq name is a shortened for Thermophilus aquaticus, a thermophilic bacteria that is the source of the particular DNA polymerase enzyme. The enzyme heat resistant property is desired because it could withstand the high temperature during the PCR process. -Kaitlin The Taq name is a shortened for Thermophilus aquaticus, a thermophilic bacteria that is the source of the particular DNA polymerase enzyme. The enzyme heat resistant property is desired because it could withstand the high temperature during the PCR process. -Kaitlin
Why taq polymerase is strored at very low temperature as it is thermostable?
to store taq DNA polymerase at low temperature is to make it viable or we can say inactive for sometime. it doesnt die or decay but remains viable.
Why is a thermostable polymerase used in PCR?
The thermostable polymerase (or Taq polymerase) is a thermostable DNA polymerase (named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated by Thomas D. Brock in 1965), is often abbreviated to "Taq Pol" (or simply "Taq"), and is frequently used in polymerase chain reaction (PCR). Taq polymerase is as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR; Therefore it replaced the DNA polymerase from E. coli originally used in PCR. Taq's optimum temperature for activity is 75-80°C, with a half-life of greater than 2 hours at 92.5°C, 40 minutes at 95°C and 9 minutes at 97.5°C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72°C.
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
Is vent polymerase a thermostable enzyme?
Yes. Vent polymerase is thermostable and is commonly used for PCR
Could you use human polymerase for the extension step in the PCR reaction?
Actually the problem with the Human polymerase is the sensitivity to temperature if we talk about PCR. That is the reason why we use Taq DNA polymerase which is thermostable where as use of human polymerase may result in loss of its function due to high temperature.
Role of Taq polymerase in PCR?
taq polymerase is special because it is very stable at high temperatures and will not denature even at the 90 degree step of pcr. taq polymerase is so heat stable because it was extracted from the bacterium thermus aquaticus, which is found in hot springs and geezers
What is the significance of thermostable polymerase in DNA technology?
It is used in Polymerase Chain Reaction (PCR), which embrace temperature of 94°C - the polymerase has to be able to sustain such temperature.
Who is the discoverer of Taq DNA polymerase?
Thomas D. Brock, An American Microbiologist
What is the source of the polymerase used in polymerase chain reaction?
Taq polymerase, the enzyme used frequently in Polymerase Chain Reaction, is extracted from Thermophilus aquaticus, a thermophilic bacteria.
Taq polymerase enzyme 500 units per ml means how to diluted this enzyme?
1 ml
What is MgCl used for in the polymerase chain reaction?
used as a co-factor o th enzime taq polimerase
What does DNA polymerases use as their substrate?
The main substrate for for Taq polymerase is magnesium chloride
