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What is the recommended extension time for Taq polymerase in PCR amplification?
The recommended extension time for Taq polymerase in PCR amplification is typically 1 minute per kilobase of DNA being amplified.
Why taq polymerase is strored at very low temperature as it is thermostable?
Storing Taq polymerase at a very low temperature (typically -20°C) helps preserve its activity over time. While Taq polymerase is thermostable and can withstand high temperatures during PCR, storing it at low temperatures helps prevent degradation and denaturation of the enzyme, leading to better performance in PCR reactions.
Dna polymerase has a what?
DNA polymerase has a proofreading function that helps to ensure accuracy during DNA replication by checking for errors and correcting them in real time.
Does RNA polymerase read 3 to 5 nucleotides at a time during transcription?
Yes, RNA polymerase reads and adds nucleotides in the 3' to 5' direction during transcription, adding them one at a time to the growing RNA strand.
What is pcr and its application?
PCR or polymerase chain reaction is a method to amplify a fragment of DNA. PCR reaction contains template DNA, primers, dNTPs, polymerase enzyme, buffer and water. The thermocycler manage the heat and time to synthesize DNA (denaturation, annealing and extension). The main application is one can amplify the gene or DNA of interest to millions of copies by using this prior to cloning.
What is the recommended extension time for Taq polymerase in PCR amplification?
The recommended extension time for Taq polymerase in PCR amplification is typically 1 minute per kilobase of DNA being amplified.
Why taq polymerase is strored at very low temperature as it is thermostable?
Storing Taq polymerase at a very low temperature (typically -20°C) helps preserve its activity over time. While Taq polymerase is thermostable and can withstand high temperatures during PCR, storing it at low temperatures helps prevent degradation and denaturation of the enzyme, leading to better performance in PCR reactions.
What if defendants request a time extension from the plaintiff in a Civil Action?
Motion for extension of time or motion for enlargement of time
Dna polymerase has a what?
DNA polymerase has a proofreading function that helps to ensure accuracy during DNA replication by checking for errors and correcting them in real time.
Which enzyme is responsible for making a polymer of DNA by adding one nucleotide monomer at a time?
DNA polymerase is the enzyme responsible for adding nucleotide monomers one at a time to the growing DNA strand during replication.
Does RNA polymerase read 3 to 5 nucleotides at a time during transcription?
Yes, RNA polymerase reads and adds nucleotides in the 3' to 5' direction during transcription, adding them one at a time to the growing RNA strand.
When the executrix files for extension because they need more time to meet the demands of a will how do you find out about information regarding that extension and is that extension indefinite?
Ask the executor or ask the clerk of the court where she filed for an extension.
What is the difference between an extension and an increase in supply?
Extension in supply is the extension of the vendor contract for a longer duration while increase is the increase within the stipulated time.
If you expect a tax refund do you need to file an extension?
If you are due a refund it is not necessary to file for an extension to file your income tax return. An extension of time to file your income tax return is NOT an extension of time to pay any amount of income tax that would be due April 15 2010.
What extension is there for gift tax when the donor obtain an automatic filing extension for income taxes?
When filing an extension for income taxes related to gift taxes, you are granted an automatic extension. As long as the paperwork is filed on time, an automatic 6 month extension is given.
What is the definition of the word extension?
The term 'extension' is defined as to enlarge, prolong, or continue. If one was to receive an extension on a term paper, one would be given more time to complete it.
How does PCR work?
This is a method of cloning DNA. So one can take a small amount of DNA and replicate it to produce an abundance. It occurs in three steps: Denature (94oC): this step break the hydrogen bond, and separates the double strand. Annealing (55-60oC): this step allows the binding of primers Extension(70oC): this is the portion that replicates the DNA These step repeat, each time constitutes a cycle. The number of DNA replicated = 2n where n equals the number of cycles
