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What is the recommended extension time for the Taq polymerase in PCR reactions?
The recommended extension time for Taq polymerase in PCR reactions is typically 1 minute per kilobase of DNA being amplified.
How are replication and pcr similar?
Both replication and PCR involve the amplification of DNA. Replication is the natural process by which cells make copies of their DNA, while PCR (polymerase chain reaction) is a laboratory technique that can make millions of copies of a specific DNA fragment in a short amount of time. Both processes require a DNA polymerase enzyme to catalyze the synthesis of new DNA strands.
Dna polymerase has a what?
DNA polymerase has a proofreading function that helps to ensure accuracy during DNA replication by checking for errors and correcting them in real time.
Does RNA polymerase read 3 to 5 nucleotides at a time during transcription?
Yes, RNA polymerase reads and adds nucleotides in the 3' to 5' direction during transcription, adding them one at a time to the growing RNA strand.
What is pcr and its application?
PCR or polymerase chain reaction is a method to amplify a fragment of DNA. PCR reaction contains template DNA, primers, dNTPs, polymerase enzyme, buffer and water. The thermocycler manage the heat and time to synthesize DNA (denaturation, annealing and extension). The main application is one can amplify the gene or DNA of interest to millions of copies by using this prior to cloning.
What is the recommended extension time for the Taq polymerase in PCR reactions?
The recommended extension time for Taq polymerase in PCR reactions is typically 1 minute per kilobase of DNA being amplified.
Is the measure of PCR?
The measure of PCR (Polymerase Chain Reaction) typically refers to the amplification of specific DNA sequences, allowing for the detection and quantification of genetic material. PCR measures the increase in DNA concentration through cycles of denaturation, annealing, and extension, which can be monitored in real-time using fluorescent dyes or probes. This technique is widely used in various applications, including medical diagnostics, forensic analysis, and genetic research.
How are replication and pcr similar?
Both replication and PCR involve the amplification of DNA. Replication is the natural process by which cells make copies of their DNA, while PCR (polymerase chain reaction) is a laboratory technique that can make millions of copies of a specific DNA fragment in a short amount of time. Both processes require a DNA polymerase enzyme to catalyze the synthesis of new DNA strands.
What is QRT-PCR?
Quantitative Reverse Transcription Polymerase Chain Reaction (QRT-PCR) is a laboratory technique used to amplify and quantify RNA. It involves reverse transcription of RNA into complementary DNA (cDNA), followed by PCR amplification of the cDNA, allowing for the measurement of gene expression levels. This method is highly sensitive and enables real-time monitoring of the amplification process, making it valuable in research and clinical diagnostics for detecting and quantifying specific RNA sequences.
What if defendants request a time extension from the plaintiff in a Civil Action?
Motion for extension of time or motion for enlargement of time
Dna polymerase has a what?
DNA polymerase has a proofreading function that helps to ensure accuracy during DNA replication by checking for errors and correcting them in real time.
Which enzyme is responsible for making a polymer of DNA by adding one nucleotide monomer at a time?
DNA polymerase is the enzyme responsible for adding nucleotide monomers one at a time to the growing DNA strand during replication.
Does RNA polymerase read 3 to 5 nucleotides at a time during transcription?
Yes, RNA polymerase reads and adds nucleotides in the 3' to 5' direction during transcription, adding them one at a time to the growing RNA strand.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
When the executrix files for extension because they need more time to meet the demands of a will how do you find out about information regarding that extension and is that extension indefinite?
Ask the executor or ask the clerk of the court where she filed for an extension.
What is the difference between an extension and an increase in supply?
Extension in supply is the extension of the vendor contract for a longer duration while increase is the increase within the stipulated time.
How does Sybr Green work in detecting DNA amplification?
Sybr Green is a fluorescent dye that binds to double-stranded DNA during the amplification process. When DNA is amplified, more double-stranded DNA is produced, causing an increase in Sybr Green fluorescence. This fluorescence can be measured and used to monitor the progress of DNA amplification in real-time.
