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Why do you use buffers in separation of serum proteins by electrophoresis?
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Plasma minus clotting proteins?
Serum
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
What do activators do?
They help the blood sample in the tube clot so that serum can be separated for analysis. Serum is the preferred specimen for many tests such as hormones, proteins and electrolytes.
What is an antitrypsin?
An antitrypsin is any of a class of serum proteins which inhibit trypsin and similar proteolytic enzymes.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What has the author Neil S Harris written?
Neil S. Harris has written: 'Multiple myeloma and related serum protein disorders' -- subject(s): Electrophoresis, Myeloma Proteins, Analysis, Blood Proteins, Blood Protein Electrophoresis, Multiple Myeloma, Blood
Plasma minus clotting proteins?
Serum
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
Purpose of Bovine serum albumin separation?
Bovine Serum Albumin (BSA) is used in cell culture media. Animal cells require serum proteins in order to grow outside the body. If we want to purify a specific protein from these cultured cells, the large amount of BSA present in the media poses a major problem in terms of contamination. This is why BSA has to be separated from the cultured cells before beginning the protein separation process.
Why did Arne Wilhelm Kaurin Tiselius win The Nobel Prize in Chemistry in 1948?
The Nobel Prize in Chemistry 1948 was awarded to Arne Tiselius for his research on electrophoresis and adsorption analysis, especially for his discoveries concerning the complex nature of the serum proteins.
How long does it take to get results from serum protein electrophoresis test?
According to webmd.com, it takes 2-3 days.
Why is destaining done after staining in agarose gel serum electrophoresis?
To remove extra Etbr and higher background fluorescence.
What causes plasma cells to produce antibodies?
Yes. Blood plasma is blood serum with the fibrinogens. Serum includes all proteins not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances (e.g., drugs and microorganisms).
When the proteins are removed from plasma the remaining fluid is called what?
serum
Serum is plasma minus the?
clotting proteins and cells
What are the predominant regulators of circulating blood volume?
Plasma proteins are the predominant regulators of circulating blood volume. Plasma proteins are also called blood proteins or serum proteins.
