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Q: Why do you wrap an agar plate in paper before incubation?
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Prewarm agar plates?

before bacterial culture, the media containing agar i.e. solid agar should dry in the incubator, that is prewarm agar plate.


How will you check the sterility of the media?

Agar is a medium, so you are checking the sterility of the agar. After preparation one usually places an agar plate at room temperature and another agar plate at 35 to 37 degrees C. After 24-48 hrs of incubation a visual check is made to see if there is any visible growth on the uninoculated plates. If you are adding blood, etc. to the agar, those components can be checked by subbing them to a blood agar plate to see if there is any growth--which would indicate non-sterile components.


Where should a label be written on an agar plate?

On the base of the agar plate.


Where should a label written on an agar plate?

On the base of the agar plate.


What does it mean to inoculate an agar plate?

to inoculate an agar plate you would place bacteria on the agar to grow


What conclusions may be drawn if no growth appeared on MacConkey agar and emb agar after inoculation of the media and an incubation period?

The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.


What conclusion may be drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period?

The conclusion drawn if no growth appeared on MacConkey agar and EMB agar after inoculation of the media and an incubation period could be the bacteria used was possibly a Gram positive non-enteric sample.


Why does petri plate is inverted during incubation?

Because during incubation moisture will form at the top of the petri dish. Inverting the dish prevents it from dropping into whatever you have in the petri dish.


How are Petri dishes sterilized?

To determine that an agar plate is sterlize you can keep it overnight in incubator without streaking and if there is any growth peresent in it then it is contaminated and if not then it is obveiously sterlize


What is a Mac plate?

MacConkey Agar plate


What is the name of the streak when you apply specimen to agar plate?

It is called a streak plate, inoculating the agar plate with a zig-zag motion.


Describe the aseptic technique that would be used when flooding the agar plate with bacteria?

An agar plate was flooded with a culture of a species of bacterium usually found in the mouth. Four steriled paper discs, A, B, C, and D, each containing a different brand of mouthwash, were then placed on the agar plate. The drawing shows the appearance of the plate after it had been incubated below body temperature for three days, this is to ensure that the bacteria are not harmful to humans. Describe the aseptic technique that would be used when flooding the agar plate with bacteria